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Literature summary extracted from

  • Breer, K.; Girstun, A.; Wielgus-Kutrowska, B.; Staro?, K.; Bzowska, A.
    Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP) (2008), Protein Expr. Purif., 61, 122-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
 Bos taurus
2.4.2.1 guanine
-
 Bos taurus
2.4.2.1 hypoxanthine
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0134
-
 Inosine pH 7.0, 25°C, recombinant enzyme Bos taurus
2.4.2.1 0.0168
-
 7-methylguanosine pH 7.0, 25°C, recombinant enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus P55859
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 0.0307
-
 inosine as a variable substrate Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methylguanosine + phosphate
-
 Bos taurus 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.0000033
-
 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine pH 7.0, 25°C, in the presence of 1 mM phosphate Bos taurus
2.4.2.1 0.0016
-
 hypoxanthine pH 7.0, 25°C Bos taurus
2.4.2.1 0.0025
-
 guanine pH 7.0, 25°C Bos taurus