EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.18 | expression in Pichia pastoris | Thermothelomyces myriococcoides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.075 | - |
cellobiose | 2,6-dichloroindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.14 | - |
lactose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 8.86 | - |
maltose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 21.5 | - |
cytochrome c | lactose as electron donor, at 40°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 140.4 | - |
2,6-dichloroindophenol | lactose as electron donor, at 40°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 157.1 | - |
glucose | 2,6-diclorindophenol as electron acceptor, at 40°C | Thermothelomyces myriococcoides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 100000 | - |
SDS-PAGE | Thermothelomyces myriococcoides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Thermothelomyces myriococcoides | - |
- |
- |
1.1.99.18 | Thermothelomyces myriococcoides CBS 208.89 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | by hi-trap column and phenyl HIC column chromatography, deglycosylation | Thermothelomyces myriococcoides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 7.4 | - |
HIC-column purified | Thermothelomyces myriococcoides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
1.1.99.18 | D-glucose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
1.1.99.18 | lactose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
1.1.99.18 | lactose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
1.1.99.18 | lactose + cytochrome c | - |
Thermothelomyces myriococcoides | ? | - |
? | |
1.1.99.18 | lactose + cytochrome c | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? | |
1.1.99.18 | maltose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides | ? | - |
? | |
1.1.99.18 | maltose + 2,6-dichloroindophenol | - |
Thermothelomyces myriococcoides CBS 208.89 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.18 | cellobiose dehydrogenase | CDH | Thermothelomyces myriococcoides |
1.1.99.18 | MtCDH | - |
Thermothelomyces myriococcoides |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 60 | - |
lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 45 | 55 | stable for 50 h | Thermothelomyces myriococcoides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4.5 | - |
lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |
1.1.99.18 | 5 | - |
lactose as electron donor, cytochrome c as electron acceptor | Thermothelomyces myriococcoides |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4 | 7 | lactose as electron donor, 2,6-diclorindophenol as electron acceptor | Thermothelomyces myriococcoides |