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Literature summary extracted from

  • Wu, C.; Wang, Y.; Zou, M.; Shan, Y.; Yao, G.; Wei, P.; Chen, G.; Wang, J.; Xu, D.
    Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14 (2007), Protein Expr. Purif., 56, 1-7.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.41 molecular biology polyclonal rabbit anti-GalNAc-T14 IgG (1: 1000 in Western blot, titer: appr. 1: 16000) for study of expression and distribution of human GalNAc-T14 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells Homo sapiens
2.4.1.41 full-length GalNAc-T14 in pFlag-CMV2, expression in HEK293T cells Homo sapiens
2.4.1.41 full-length GalNAc-T14 in pGEX-4T-1, expression in Escherichia coli BL21(DE3) Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 Golgi apparatus
-
Homo sapiens 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 61000
-
endogenous GalNAc-T14 in HEK293T cells detected by anti-GalNAc-T14 antibody Homo sapiens
2.4.1.41 61000
-
Flag-tag fusion protein expressed in HEK293T cells detected by anti-Flag and anti-GalNAc-T14 antibody Homo sapiens
2.4.1.41 87000
-
SDS-PAGE Homo sapiens
2.4.1.41 87000
-
bacterially expressed GST-fusion protein (GST: 26 kDa) detected by anti-GST and anti-GalNAc-T14 antibody Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Homo sapiens mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Homo sapiens Q96FL9
-
-
2.4.1.41 Homo sapiens Q96FL9 GalNAc-T14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 GST fusion protein purified by affinity chromatography, elution with 10 mM glutathione, pH8 Homo sapiens
2.4.1.41 GSTrap FF chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 brain
-
Homo sapiens
-
2.4.1.41 brain cDNA library used for cloning Homo sapiens
-
2.4.1.41 HEK-293T cell expression detected by Western blot Homo sapiens
-
2.4.1.41 MCF-7 cell no expression detected by Western blot Homo sapiens
-
2.4.1.41 renal cell carcinoma cell RCC 7860, expression detected by Western blot Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide
-
Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 GalNAc-T
-
Homo sapiens
2.4.1.41 GalNAc-T14
-
Homo sapiens
2.4.1.41 polypeptide N-acetylgalactosaminyltransferase
-
Homo sapiens
2.4.1.41 polypeptide N-acetylgalactosaminyltransferase 14
-
Homo sapiens