EC Number | Cloned (Comment) | Organism |
---|---|---|
7.1.2.2 | expression of the soluble His3-tagged V1VO ATPase subunit A of in Escherichia coli | Aedes albopictus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.1.2.2 | Mg2+ | activates | Aedes albopictus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.1.2.2 | 62800 | - |
x * 62800, recombinant His3-tagged subunit A of V1VO ATPase, SDS-PAGE | Aedes albopictus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.2.2 | ATP + H2O + H+/in | Aedes albopictus | - |
ADP + phosphate + H+/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.2.2 | Aedes albopictus | - |
Asian tiger mosquito | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.1.2.2 | recombinant soluble His3-tagged V1VO ATPase subunit A from Escherichia coli by immobilized metal affinity and ion exchange chromatography to homogeneity | Aedes albopictus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.2.2 | ATP + H2O + H+/in | - |
Aedes albopictus | ADP + phosphate + H+/out | - |
? | |
7.1.2.2 | ATP + H2O + H+/in | ADP-analogue ADP-ATTO-647N bind slightly weaker to subunit A than the ATP-analogue ATP-ATTO-647N, binding of different nucleotides cause different secondary structural alterations in this subunit | Aedes albopictus | ADP + phosphate + H+/out | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.1.2.2 | ? | x * 62800, recombinant His3-tagged subunit A of V1VO ATPase, SDS-PAGE | Aedes albopictus |
7.1.2.2 | More | secondary structure analysis by circular dichroism spectroscopy shows that subunit A of V1VO ATPase comprises 43% alpha-helix, 25% beta-sheet and 40% random coil content | Aedes albopictus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.2.2 | V1VO ATPase | - |
Aedes albopictus |