EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.112 | EDTA | increase in activity about 40% at 10 mM | Homo sapiens | |
3.4.21.112 | EGTA | increase in activity about 40% at 10 mM | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.112 | first 997 amino acids of human S1P lacking the C-terminal transmembrane region. Full-length sequence of human S1P. Both tagged with a C-terminal 8 x His-tag, subcloned into the entry vector pDONR201 and expressed by baculovirus expression vector system in Sf9 cells. Additionally, first 997 amino acids of human S1P lacking the C-terminal transmembrane region expressed in Sf21 cells and Trichoplusia ni egg, High Five cells | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.112 | 3,4-dichloroisocoumarin | potent slow binding inhibitor, 100% inhibition by 0.05 mM | Homo sapiens | |
3.4.21.112 | Co2+ | - |
Homo sapiens | |
3.4.21.112 | decanoyl-RVKR-chlorometylketone | 66% inhibition at 0.05 mM | Homo sapiens | |
3.4.21.112 | gabexate mesylate | 8% inhibition at 0.05 mM | Homo sapiens | |
3.4.21.112 | additional information | not inhibited by Mn2+, 7B2 C-terminal peptide, ProSAAS235-244 peptide, ProSAAS235-246 peptide, trypsin inhibitor from bovine pancreas, trypsin inhibitor from soybean, Pefabloc SC (4-(2-aminoethyl) benzene sulphonyl fluoride), benzamidine, p-aminobenzamidine and chymostatin | Homo sapiens | |
3.4.21.112 | Ni2+ | - |
Homo sapiens | |
3.4.21.112 | PMSF | 12% inhibition at 0.05 mM | Homo sapiens | |
3.4.21.112 | Zn2+ | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.112 | 0.0031 | - |
Abz-DIYISRRLLGTFTY(NO2)A | - |
Homo sapiens | |
3.4.21.112 | 0.0255 | - |
Ac-Arg-Arg-Leu-Leu-p-nitroanilide | - |
Homo sapiens | |
3.4.21.112 | 0.1335 | - |
Ac-Arg-Ser-Leu-Lys-p-nitroanilide | - |
Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.112 | CaCl2 | recombinant S1P-1 activity can be stimulated by Ca2+, but is not dependent on Ca2+. Increases activity of recombinant S1P-1 60% at 4 mM, higher concentrations of CaCl2 lowers the activity | Homo sapiens | |
3.4.21.112 | K-phosphate buffer | - |
Homo sapiens | |
3.4.21.112 | KCl | increases activity of recombinant S1P-1 25% dependent on the substrate used | Homo sapiens | |
3.4.21.112 | Na-phosphate buffer | - |
Homo sapiens | |
3.4.21.112 | NaCl | increases activity of recombinant S1P-1 25% at 50 mM dependent on the substrate used | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.112 | 91000 | - |
gel filtration | Homo sapiens |
3.4.21.112 | 92300 | - |
sequence analysis | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.112 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.112 | first 997 amino acids of human S1P lacking the C-terminal transmembrane region 103fold purified by immobilised metal affinity chromatography on Co2+ column | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.112 | 0.007 | - |
first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Trichoplusia ni egg, High Five cells | Homo sapiens |
3.4.21.112 | 0.022 | - |
first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Sf21 cells, 103fold purified | Homo sapiens |
3.4.21.112 | 0.2 | - |
first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Sf21 cells | Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.112 | -80°C, 40% (v/v) glycerol | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.112 | Abz-DIYISRRLLGTFTY(NO2)A + H2O | S1P displays pronounced positive cooperativity with this substrate derived from the viral coat glycoprotein of the lassa virus | Homo sapiens | ? | - |
? | |
3.4.21.112 | Ac-Arg-Arg-Leu-Leu-p-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.112 | Ac-Arg-Ser-Leu-Lys-p-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.112 | Ac-Leu-4-methyl-coumaryl-7-amide + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.112 | MEROPS S08.8063 | - |
Homo sapiens |
3.4.21.112 | S1P | - |
Homo sapiens |
3.4.21.112 | SKI-1 | - |
Homo sapiens |
3.4.21.112 | subtilisin/kexin isozyme 1 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.112 | 0.005 | - |
Ac-Arg-Ser-Leu-Lys-p-nitroanilide | - |
Homo sapiens | |
3.4.21.112 | 0.03 | - |
Ac-Arg-Arg-Leu-Leu-p-nitroanilide | - |
Homo sapiens | |
3.4.21.112 | 0.046 | - |
Abz-DIYISRRLLGTFTY(NO2)A | - |
Homo sapiens |