EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.62 | Escherichia coli HB101 transformed with plasmid pBR1560 containing the wild-type prepro-subtilisin. G56S-propeptide and His-tagged G56S-propeptide overexpressed in Escherichia coli BL21-CodonPlus(DE3)-RIL transformed with vector pET25b and in Escherichia coli BL21(DE3) with vector pET28a, respectively | Thermococcus kodakarensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.21.62 | E61G | pro-subtilisin mutant, exhibits a halo-forming activity at 80, 70 and 60°C | Thermococcus kodakarensis |
3.4.21.62 | E61K | pro-subtilisin mutant, exhibits a halo-forming activity at 80, 70 and 60°C | Thermococcus kodakarensis |
3.4.21.62 | G56S | pro-subtilisin mutant, does not seriously affect the structure of pro-subtilisin. Is more effectively matured than pro-subtilisin at lower temperatures | Thermococcus kodakarensis |
3.4.21.62 | G56S/T135S | low-temperature adaptation of prepro-G56S/T135S-subtilisin | Thermococcus kodakarensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.62 | additional information | enzymatic activity of mature subtilisin is inhibited by G56S-propeptide and wild-type propeptide in a concentration-dependent manner | Thermococcus kodakarensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.62 | Ca2+ | - |
Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.62 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.62 | G56S-propeptide and His-tagged G56S-propeptide purified by gel filtration | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.62 | azocasein + H2O | - |
Thermococcus kodakarensis | ? | - |
? | |
3.4.21.62 | N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | - |
Thermococcus kodakarensis | N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.62 | 20 | 80 | G56S-propeptide is almost completely degraded within 510 min at 80°C, 1530 min at 60°C and 3 h at 40°C, but is not completely degraded upon incubation for 3 h at 20°C. Pro-subtilisin is rapidly autoprocessed into propeptide and mature subtilisin within 1 min even at 20°C. However, the subsequent degradation of propeptide proceeds much more slowly than that of G56S-propeptide. Propeptide is almost completely degraded only when it is incubated at 80°C for 30 min. It is not completely degraded for up to 3 h at 20, 40 and 60°C | Thermococcus kodakarensis |