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Literature summary extracted from

  • Tomatsu, H.; Takano, J.; Takahashi, H.; Watanabe-Takahashi, A.; Shibagaki, N.; Fujiwara, T.
    An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil (2007), Proc. Natl. Acad. Sci. USA, 104, 18807-18812.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.3.2.5 additional information MOT1 expression in roots is slightly but significantly reduced under Mo limitation Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.5 gene mot1, quantitative expression analysis by RT-PCR, expression of GFP-tagged MOT1 in Nicotiana tabacum BY-2 cells under control of the cauliflower mosaic virus 35S RNA promoter near or at the plasma membrane of BY2 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.5 additional information construction of T-DNA insertion mutants of Arabidopsis thaliana MOT1, Mo concentrations in shoots of the mot1-1 and mot1-2 mutant plants are reduced to 10% and 20%, respectively, of that in the wild-type, and, in roots, the Mo concentrations are reduced to 20% and 25% of that in the wild-type, phenotype, overview. Molybdate uptake by MOT1 in transformed yeast is not affected by coexistent sulfate, and MOT1 does not complement a sulfate transporter-deficient yeast mutant strain Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.3.2.5 additional information MOT1 expression in shoots is decreased to 50% of that in plants supplied with sufficient Mo Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.5 0.00002
-
 molybdate
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.5 plasma membrane
-
 Arabidopsis thaliana 5886
-
7.3.2.5 vesicle
-
 Arabidopsis thaliana 31982
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.5 ATP + H2O + molybdate/out Arabidopsis thaliana
-
 ADP + phosphate + molybdate/in
-
 ?
7.3.2.5 additional information Arabidopsis thaliana MOT1 is required for efficient uptake and translocation of molybdate and for normal growth under conditions of limited molybdate supply ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.5 Arabidopsis thaliana
-
 gene At2g25680 or mot1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.3.2.5 root
-
 Arabidopsis thaliana
-
7.3.2.5 shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.5 ATP + H2O + molybdate/out
-
 Arabidopsis thaliana ADP + phosphate + molybdate/in
-
 ?
7.3.2.5 additional information MOT1 is required for efficient uptake and translocation of molybdate and for normal growth under conditions of limited molybdate supply Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.5 molybdate transporter
-
 Arabidopsis thaliana
7.3.2.5 MOT1
-
 Arabidopsis thaliana