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Literature summary extracted from

  • Erb, T.J.; Berg, I.A.; Brecht, V.; Mueller, M.; Fuchs, G.; Alber, B.E.
    Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway (2007), Proc. Natl. Acad. Sci. USA, 104, 10631-10636.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.8 expressed in Escherichia coli Cereibacter sphaeroides
1.3.1.85 expressed in Escherichia coli BL21(DE3) cells Cereibacter sphaeroides 2.4.1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.85 0.2
-
 CO2 in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
1.3.1.85 0.2
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
1.3.1.85 0.4
-
 crotonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1
1.3.1.85 0.7
-
 NADPH in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.85 additional information comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals Methylorubrum extorquens AM1
1.3.1.85 additional information comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals Cereibacter sphaeroides 2.4.1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.85 47700
-
 2 * 47700, gel filtration Cereibacter sphaeroides 2.4.1
1.3.1.85 105000
-
 gel filtration Cereibacter sphaeroides 2.4.1

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.8 Cereibacter sphaeroides
-
-
-
1.3.1.85 Cereibacter sphaeroides 2.4.1
-
-
-
1.3.1.85 Methylorubrum extorquens AM1
-
-
-
1.3.1.85 Streptomyces coelicolor
-
 strain A3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.8 DEAE-Sepharose column chromatography and Cibacron Blue column chromatography Cereibacter sphaeroides
1.3.1.85 DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography Cereibacter sphaeroides 2.4.1

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.85 culture condition:acetate-grown cell
-
 Cereibacter sphaeroides 2.4.1
-
1.3.1.85 culture condition:butyrate-grown cell
-
 Streptomyces coelicolor
-
1.3.1.85 culture condition:methanol-grown cell
-
 Methylorubrum extorquens AM1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.8 crotonyl-CoA + NADPH
-
 Cereibacter sphaeroides butanoyl-CoA + NADP+
-
 ?
1.3.1.8 additional information no activity with acetoacetyl-CoA or (R)-3-hydroxybutyryl-CoA Cereibacter sphaeroides ?
-
 ?
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Streptomyces coelicolor (2S)-ethylmalonyl-CoA + NADP+
-
 r
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Methylorubrum extorquens AM1 (2S)-ethylmalonyl-CoA + NADP+
-
 r
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+ crotonyl-CoA is the best substrate Cereibacter sphaeroides 2.4.1 (2S)-ethylmalonyl-CoA + NADP+
-
 r
1.3.1.85 crotonyl-CoA + NADPH + H+ reduction at low rate Streptomyces coelicolor butyryl-CoA + NADP+
-
 ir
1.3.1.85 crotonyl-CoA + NADPH + H+ reduction at low rate Methylorubrum extorquens AM1 butyryl-CoA + NADP+
-
 ir
1.3.1.85 crotonyl-CoA + NADPH + H+ reduction at low rate Cereibacter sphaeroides 2.4.1 butyryl-CoA + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.3.1.85 homodimer 2 * 47700, gel filtration Cereibacter sphaeroides 2.4.1

Synonyms

EC Number Synonyms Comment Organism
1.3.1.8 crotonyl-CoA reductase
-
 Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.85 104
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.8 NADPH
-
 Cereibacter sphaeroides
1.3.1.85 NADPH
-
 Streptomyces coelicolor
1.3.1.85 NADPH
-
 Methylorubrum extorquens AM1
1.3.1.85 NADPH
-
 Cereibacter sphaeroides 2.4.1

General Information

EC Number General Information Comment Organism
1.3.1.85 metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Streptomyces coelicolor
1.3.1.85 metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Methylorubrum extorquens AM1
1.3.1.85 metabolism crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway Cereibacter sphaeroides 2.4.1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.85 520
-
 (2S)-ethylmalonyl-CoA in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication Cereibacter sphaeroides 2.4.1