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Literature summary extracted from

  • Arnell, R.; Johannisson, R.; Lindholm, J.; Fornstedt, T.; Ersson, B.; Ballagi, A.; Caldwell, K.
    Biotechnological approach to the synthesis of 9alpha-hydroxylated steroids (2007), Prep. Biochem. Biotechnol., 37, 309-321.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.24 DNA and amino acid sequenc determination, expression in Escherichia coli strain BL21 Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.24 progesterone + AH2 + O2 Mycolicibacterium smegmatis reaction with whole cells in a bioreactor 9alpha-hydroxyprogesterone + A + H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.24 Mycolicibacterium smegmatis
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.24 recombinant enzyme from Escherichia coli by immobilized metal affinity and ion exchange chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.24 4-androsten-3,17-dione + AH2 + O2
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 Mycolicibacterium smegmatis ?
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 ?
1.14.99.24 additional information product determination and identification by LLE, HPLC-DAD, MS, and NMR, overview Mycolicibacterium smegmatis ?
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 ?
1.14.99.24 progesterone + AH2 + O2 reaction with whole cells in a bioreactor Mycolicibacterium smegmatis 9alpha-hydroxyprogesterone + A + H2O
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 ?
1.14.99.24 progesterone + AH2 + O2 reaction with whole cells in a bioreactor, low in vitro activity Mycolicibacterium smegmatis 9alpha-hydroxyprogesterone + A + H2O
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.24 steroid 9alpha-hydroxylase
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 Mycolicibacterium smegmatis