Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Szwengiel, A.; Czarnecka, M.; Czarnecki, Z.
    Levan synthesis during associated action of levansucrase and Candida cacaoi DSM 2226 yeast (2007), Pol. J. Food Nutr. Sci., 57, 433-440.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.10 synthesis D-glucose acts as an inhibitor of the transfructosylation reaction, Candida cacaoi selectively removes glucose from the reaction medium, the decrease of glucose concentration by yeast in the medium by 16-19% results in the increased degree of levan polymerisation (by 6 to 9%) and efficiency of levan synthesis (by 9 to 11%) Bacillus subtilis

General Stability

EC Number General Stability Organism
2.4.1.10 levansucrase shows high stability during 140 h synthesis at 37°C Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 D-glucose D-glucose acts as an inhibitor of the transfructosylation reaction, the decrease of glucose concentration by yeast in the medium by 16-19% results in the increased degree of levan polymerisation (by 6 to 9%) and efficiency of levan synthesis (by 9 to 11%) Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.10 Mn2+ The transferase activity of levansucrase in the reaction mixture supplemented with Mn2+ is 100% higher than the enzyme activity in medium without metal ions, the hydrolytic activity of the levansucrase is lowered by 80% Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus subtilis
-
 DSM 347
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.10 culture supernatant ethanol precipitation Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64% Bacillus subtilis D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?