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Literature summary extracted from

  • Raso, M.J.; Munoz, A.; Pineda, M.; Piedras, P.
    Biochemical characterization of an allantoate-degrading enzyme from French bean (Phaseolus vulgaris): the requirement of phenylhydrazine (2007), Planta, 226, 1333-1342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.9 2,4-Dinitrophenylhydrazine can partially substitute for phenylhydrazine, 67% of the activity with phenylhydrazine Phaseolus vulgaris
3.5.3.9 L-arginine can partially substitute for phenylhydrazine, 19% of the activity with phenylhydrazine Phaseolus vulgaris
3.5.3.9 L-lysine can partially substitute for phenylhydrazine, 29% of the activity with phenylhydrazine Phaseolus vulgaris
3.5.3.9 phenylhydrazine completely dependent on phenylhydrazine, which acts as an activator at low concentrations and decreases the affinity of the enzyme for the substrate at higher concentrations, the activation is reduced by pyridoxamine-5'-phosphate and pyridoxal-5'-phosphate Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.9 2-mercaptoethanol 21% inhibition at 1 mM Phaseolus vulgaris
3.5.3.9 Borate 8% inhibition at 10 mM Phaseolus vulgaris
3.5.3.9 EDTA complete inhibition at 10 mM, 85% inhibition at 5 mM, the activity is partially restored only by Mn2+, activity inhibited by EDTA is not recovered after EDTA removal by dialysis Phaseolus vulgaris
3.5.3.9 hydroxylamine 67% inhibition at 1 mM Phaseolus vulgaris
3.5.3.9 L-Asp 29% inhibition at 10 mM Phaseolus vulgaris
3.5.3.9 NEM 15% inhibition at 5 mM Phaseolus vulgaris
3.5.3.9 nitrate inhibits the enzyme, overview Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.9 Mn2+ required Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.9 allantoate + 2 H2O Phaseolus vulgaris allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation ureidoglycolate + 2 NH3 + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.9 Phaseolus vulgaris
-
 cv. Great Northern
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.9 native enzyme 22.4fold by ultracentrifugation and batch separation using an anion exchanger resin Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.9 additional information the activity is lower in extracts obtained from leaves and fruits of French bean grown with nitrate than in plants actively fixing nitrogen Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.9 0.365
-
 partially purified enzyme Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.5.3.9 -20°C, partially purified enzyme, retains the activity for 11 days Phaseolus vulgaris
3.5.3.9 4°C, partially purified enzyme, retains the activity for 4 days Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.9 allantoate + 2 H2O
-
 Phaseolus vulgaris ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 allantoate + 2 H2O allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation Phaseolus vulgaris ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 additional information no activity with ureidoglycolate Phaseolus vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.9 allantoate-degrading enzyme
-
 Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.9 35
-
-
 Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.9 65
-
 30 min, inactivation Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.9 7
-
 activity is maximal in the buffers triethanolamine, TES, HEPES, or phosphate, and 25% lower in MES buffer Phaseolus vulgaris