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Literature summary extracted from

  • Zhang, F.; Niu, B.; Wang, Y.; Chen, F.; Wang, S.; Xu, Y.; Jiang, L.; Gao, S.; Wu, J.; Tang, L.; Jia, Y.
    A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress (2008), Plant Sci., 174, 510-518.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.8 NaCl Expression of JcBD1 protein under different salt concentrations increased clearly in recombinant plasmid transgenic cells at the presence of 100-400 mM NaCl Jatropha curcas

Application

EC Number Application Comment Organism
1.2.1.8 energy production the seed of the plant is the raw material for biodiesels Jatropha curcas

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 expressed in Escherichia coli BL21 Jatropha curcas

General Stability

EC Number General Stability Organism
1.2.1.8 total RNA of leaves subjected to RT-PCR analysis to examine the influence of environmental stress like drought, salt or heat on BADH expression. Results showed tha JcBD1 gene transcripts of those plants exposed to 30% PEG, 300 mM NaCl or 50°C heat stress were obviously higher than that of the control plants. JcBD1 mRNA levels in stress treated leaved are increased by 79% or more, as compared with the control, which strongly confirms that mRNA expression are relative to abiotic stress. Jatropha curcas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.8 Cl- BADH plays an important role in the tolerance of plants to salinity Jatropha curcas
1.2.1.8 Na+ BADH plays an important role in the tolerance of plants to salinity Jatropha curcas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 80000
-
 Expressed protein analyzed by SDS–PAGE Jatropha curcas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Jatropha curcas
-
 glycine betaine + NADH + H+ glycine betaine is not only an nontoxic osmoprotectant but also maintains protein and membrane conformations under various stress conditions ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Jatropha curcas B2BBY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 For purification of the recombinant protein, cells of 500 ml culture are harvested. Pellet is suspended in lysis buffer and suspension was incubated. Following sonication, suspension is centrifuged. Clear supernatant is collected and loaded onto a Glutathione-Agarose column equilibrated with buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA). The recombinant protein is eluted with elution buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA, and 1.0 mM reduced glutathione). Eluted protein is collected and the purity is analyzed by 12% SDS-PAGE. To remove the GST moiety, the recombinant proteins are digested with PreScission protease. Jatropha curcas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.8 flower found in all plant tissues excepting roots, expression level is highest in leaves and stems Jatropha curcas
-
1.2.1.8 leaf found in all plant tissues excepting roots, expression level is highest in leaves and stems Jatropha curcas
-
1.2.1.8 root find in all plant tissues excepting roots, expression level is highest in leaves and stems Jatropha curcas
-
1.2.1.8 seed found in all plant tissues excepting roots, expression level is highest in leaves and stems Jatropha curcas
-
1.2.1.8 stem found in all plant tissues excepting roots, expression level is highest in leaves and stems Jatropha curcas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 additional information
-
 activities assayed in 400 ml of reaction mixture consisting of 20 mM Tris–HCl buffer, 0.5 mM NAD+, 5 mM DTT, and 100 ml purified protein elutes solution or crude bacterial extracts. The substrate betaine aldehyde is added to the reaction mixtures after preincubation for 10 min at 37°C to start the reactions. Enzyme activities are determined according to the absorbance at 340 nm (reflecting the consumption of NAD+). One unit of the activity is defined as 1 micromol/min of NAD+ consumption. The purified recombinant protein solution and crude bacterial extracts of the transformed cells both show high enzymatic activities. The activity is 2.53 nmol/ml and 2.06 nmol/ml, respectively, whereas crude bacterial extracts of untransformed Escherichia coli is 0.68 nmol/ml Jatropha curcas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Jatropha curcas glycine betaine + NADH + H+ glycine betaine is not only an nontoxic osmoprotectant but also maintains protein and membrane conformations under various stress conditions ir

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Jatropha curcas
1.2.1.8 betaine aldehyde dehydrogenase
-
 Jatropha curcas
1.2.1.8 JcBD1
-
 Jatropha curcas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
 assay at Jatropha curcas

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Jatropha curcas

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.8 Jatropha curcas calculated from amino acid sequence
-
 5.3