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Literature summary extracted from

  • Ohkama-Ohtsu, N.; Oikawa, A.; Zhao, P.; Xiang, C.; Saito, K.; Oliver, D.J.
    A gamma-glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis (2008), Plant Physiol., 148, 1603-1613.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.9 additional information oxp1-1 and oxp1-2, oxoprolinase knockout mutants Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.13 vacuole
-
 Arabidopsis thaliana 5773
-
3.5.2.9 cytoplasm
-
 Arabidopsis thaliana 5737
-
4.3.2.9 cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 additional information Arabidopsis thaliana in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Arabidopsis thaliana
-
-
-
2.3.2.2 Arabidopsis thaliana Q9LR30
-
-
3.4.19.13 Arabidopsis thaliana Q9M0G0
-
-
3.5.2.9 Arabidopsis thaliana Q9FIZ7 wild-type plants and oxp1-1 and oxp2-2 mutants
-
4.3.2.9 Arabidopsis thaliana
-
 ggt1-1/ggt4-1 mutants
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.13 additional information isoform GGT3 is transcribed at relatively high levels in all parts of the plant. It is a major contributor to total enzymic activity in roots, but a relatively minor contributor in other tissues Arabidopsis thaliana
-
3.4.19.13 root isoform GGT3 is a major contributor to total enzymic activity in roots, but a relatively minor contributor in other tissues Arabidopsis thaliana
-
3.5.2.9 leaf
-
 Arabidopsis thaliana
-
4.3.2.9 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 additional information in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis Arabidopsis thaliana ?
-
 ?
3.4.19.13 glutathione-S-monobromobimane conjugate + H2O
-
 Arabidopsis thaliana L-cysteinylglycine-S-monobrombimane conjugate + L-glutamate
-
 ?
3.5.2.9 5-oxo-L-proline + ATP + H2O assay at 30°C, pH 9.5, reaction stopped by 20 microl acetic acid and heating for 5 min at 100°C Arabidopsis thaliana L-glutamate + ADP + phosphate
-
 ?
4.3.2.9 gamma-glutamyl-Cys + H2O assay at 30°C, pH 8.0, 60 min, 48 microl substrate, reaction stopped with 6 microl HCl Arabidopsis thaliana 5-oxoproline + L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 GGT1
-
 Arabidopsis thaliana
2.3.2.2 GGT4
-
 Arabidopsis thaliana
3.4.19.13 At4g29210 locus name Arabidopsis thaliana
3.4.19.13 GGT3
-
 Arabidopsis thaliana
3.4.19.13 GGT4
-
 Arabidopsis thaliana
3.5.2.9 5-oxoprolinase
-
 Arabidopsis thaliana
3.5.2.9 5OPase
-
 Arabidopsis thaliana
4.3.2.9 gamma-glutamyl cyclotransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.9 30
-
 assay at Arabidopsis thaliana
4.3.2.9 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.9 9.5
-
 assay at Arabidopsis thaliana
4.3.2.9 8
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.4.19.13 physiological function enzyme catalyzes the obligate initial step in glutathione conjugate metabolism. Enzyme disruption plants grown on soil under normal conditions until they set seed do not display visible differences compared with wild-type plants Arabidopsis thaliana