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Literature summary extracted from

  • von Schwartzenberg, K.; Nunez, M.F.; Blaschke, H.; Dobrev, P.I.; Novak, O.; Motyka, V.; Strnad, M.
    Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins (2007), Plant Physiol., 145, 786-800.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 expressed in Physcomitrella patens protoplasts, expression of the AtCKX2 gene results in an up to 27fold increase of activity compared with wild type Physcomitrella patens, expression of AtCKX2 results in a significant decrease of cytokinin level and morphological changes such as altered morphology of filaments, impaired budding, and loss of sexual reproductivity Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 additional information Physcomitrium patens irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde ?
-
?
1.5.99.12 additional information Arabidopsis thaliana irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Arabidopsis thaliana Q9FUJ3
-
-
1.5.99.12 Physcomitrium patens
-
Hedw., wild type and transgenic cells expressing the AtCKX2 gene from Arabidopsis thaliana, expression of AtCKX2 results in a significant decrease of cytokinin level and morphological changes such as altered morphology of filaments, impaired budding, and loss of sexual reproductivity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde Physcomitrium patens ?
-
?
1.5.99.12 additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde Arabidopsis thaliana ?
-
?
1.5.99.12 N6-(2-isopentenyl)adenine + CuCl2
-
Physcomitrium patens ?
-
?
1.5.99.12 N6-(2-isopentenyl)adenine + CuCl2
-
Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 AtCKX2
-
Arabidopsis thaliana
1.5.99.12 CKX
-
Physcomitrium patens
1.5.99.12 CKX
-
Arabidopsis thaliana
1.5.99.12 cytokinin oxidase/dehydrogenase
-
Physcomitrium patens
1.5.99.12 cytokinin oxidase/dehydrogenase
-
Arabidopsis thaliana
1.5.99.12 More formerly EC 1.4.3.18 Physcomitrium patens
1.5.99.12 More formerly EC 1.4.3.18 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD
-
Physcomitrium patens
1.5.99.12 FAD
-
Arabidopsis thaliana