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Literature summary extracted from

  • Ryan, P.R.; Liu, Q.; Sperling, P.; Dong, B.; Franke, S.; Delhaize, E.
    A higher plant DELTA8 sphingolipid desaturase with a preference for (Z)-isomer formation confers aluminum tolerance to yeast and plants (2007), Plant Physiol., 144, 1968-1977.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4 expression in Saccharomyces cerevisiae confers tolerance to aluminium to the cells Arabidopsis thaliana
1.14.19.4 gene S581, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae and constitutively in Arabidopsis thaliana, under control of the CaMV35S promoter, confers tolerance to aluminium and gadolinium, Gd3+, but not to lanthanum, La3+, or manganese, Mn2+, to cells and seedlings, respectively, the transgenic expression leads to the accumulation of 8(Z/E)-C18-phytosphingenine and 8(Z/E)-C20-phytopshingenine in yeast and to the accumulation of 8(Z/E)-C18-phytosphingenine in the leaves and roots of Arabidopsis plants, overview Stylosanthes hamata
1.14.19.29 expressed in Saccharomyces cerevisiae and Arabidopsis thaliana Stylosanthes hamata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.4 DELTA4-sphingenine + reduced acceptor + O2 Stylosanthes hamata
-
 DELTA4,8-sphingadienine + acceptor + 2 H2O production of two water molecules in the reaction ?
1.14.19.4 phytosphinganine + reduced acceptor + O2 Stylosanthes hamata
-
 DELTA8-phytosphingenine + acceptor + H2O production of two water molecules in the reaction ?
1.14.19.4 sphinganine + reduced acceptor + O2 Stylosanthes hamata
-
 DELTA8-sphingenine + acceptor + 2 H2O production of two water molecules in the reaction ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Stylosanthes hamata
-
 (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Stylosanthes hamata
-
 (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O the enzyme exhibits preference for (Z)-isomer formation ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Arabidopsis thaliana AF001394
-
-
1.14.19.4 Stylosanthes hamata A7Y7E1 gene S581
-
1.14.19.29 Stylosanthes hamata A7Y7E1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.4 root
-
 Stylosanthes hamata
-
1.14.19.29 root
-
 Stylosanthes hamata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.4 DELTA4-sphingenine + reduced acceptor + O2
-
 Stylosanthes hamata DELTA4,8-sphingadienine + acceptor + 2 H2O production of two water molecules in the reaction ?
1.14.19.4 additional information DELTA8 sphingolipid desaturase creates double bonds in long-chain bases, i.e. sphingobases, such as sphinganine, phytosphinganine, or 4-sphingenine to produce E- and Z-isomers of 8-sphingenine, 8-phytosphingenine, or 4,8-sphingadienine, respectively Stylosanthes hamata ?
-
 ?
1.14.19.4 phytosphinganine + reduced acceptor + O2
-
 Stylosanthes hamata DELTA8-phytosphingenine + acceptor + H2O production of two water molecules in the reaction ?
1.14.19.4 sphinganine + reduced acceptor + O2
-
 Stylosanthes hamata DELTA8-sphingenine + acceptor + 2 H2O production of two water molecules in the reaction ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
 Stylosanthes hamata (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
 Stylosanthes hamata (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O the enzyme exhibits preference for (Z)-isomer formation ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8 sphingobase desaturase
-
 Stylosanthes hamata
1.14.19.4 DELTA8 sphingobase desaturase
-
 Arabidopsis thaliana
1.14.19.4 Delta8 sphingolipid desaturase
-
 Stylosanthes hamata
1.14.19.4 Delta8 sphingolipid desaturase
-
 Arabidopsis thaliana
1.14.19.29 Delta8 sphingolipid desaturase
-
 Stylosanthes hamata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.29 cytochrome b5
-
 Stylosanthes hamata

General Information

EC Number General Information Comment Organism
1.14.19.29 physiological function DELTA8 sphingobase desaturase confer aluminum tolerance in seedlings Stylosanthes hamata