EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.27 | construct consisting of the 35S promoter fused to the first intron of the maize alcohol dehydrogenase gene, fused to the isoform IPT2 coding sequence and a terminator. Ectopic expression in Arabidopsis thaliana | Zea mays |
2.5.1.112 | expressed in Escherichia coli BL21-Star cells | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.27 | additional information | ectopic expression of isoform IPT2 in calli and in planta of Arabidopsis thaliana creates phenotypes consistent with cytokinin overproduction | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.112 | dimethylallyl diphosphate + ADP | Zea mays | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + AMP | Zea mays | low efficiency | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | Zea mays | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
2.5.1.112 | additional information | Zea mays | 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.27 | Zea mays | B2XWS4 | isoform isopentenyl transferase 2, IPT2 | - |
2.5.1.112 | Zea mays | - |
cultivar B73 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.112 | Talon metal affinity column chromatography | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.27 | embryo | at 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages | Zea mays | - |
2.5.1.27 | kernel | strong expression of isoform IPT2 in developing kernels. At 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages | Zea mays | - |
2.5.1.27 | leaf | very low level of isoform IPT2 protein | Zea mays | - |
2.5.1.27 | root | very low level of isoform IPT2 protein | Zea mays | - |
2.5.1.27 | stem | very low level of isoform IPT2 protein | Zea mays | - |
2.5.1.112 | embryo | - |
Zea mays | - |
2.5.1.112 | endosperm | - |
Zea mays | - |
2.5.1.112 | kernel | strong expression in developing kernel | Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.27 | dimethylallyl diphosphate + ADP | ADP and ATP are preferred over AMP | Zea mays | diphosphate + N-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + AMP | ADP and ATP are preferred over AMP | Zea mays | diphosphate + N-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + ATP | ADP and ATP are preferred over AMP | Zea mays | diphosphate + N-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
2.5.1.27 | additional information | no substrate: 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate | Zea mays | ? | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ADP | - |
Zea mays | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + AMP | low efficiency | Zea mays | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | - |
Zea mays | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
2.5.1.112 | additional information | 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.112 | IPT2 | - |
Zea mays |
2.5.1.112 | isopentenyl transferase 2 | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.112 | ADP | the enzyme preferentially uses ADP and ATP over AMP | Zea mays | |
2.5.1.112 | ATP | the enzyme preferentially uses ADP and ATP over AMP | Zea mays |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.5.1.112 | Zea mays | enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP) | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.112 | physiological function | the enzyme is expressed during kernel development | Zea mays |