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Literature summary extracted from
- Isolation and characterization of the cytochrome P450 gene CYP82E5v2 that mediates nicotine to nornicotine conversion in the green leaves of tobacco (2007), Plant Cell Physiol., 48, 1567-1574.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.B11 | expression in Saccharomyces cerevisiae | Nicotiana tabacum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.B11 | 0.056 | - |
Nicotine | pH 7.1, 23°C | Nicotiana tabacum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.B11 | microsome | - |
Nicotiana tabacum | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.B11 | Nicotiana tabacum | A9QNE6 | isoform CYP82E5v | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.B11 | leaf | high expression in green leaf | Nicotiana tabacum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.B11 | nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana tabacum | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.B11 | cytochrome P450 | - |
Nicotiana tabacum |
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Release 2023.1 (January 2023)
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