Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Keskiaho, K.; Hieta, R.; Sormunen, R.; Myllyharju, J.
    Chlamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly (2007), Plant Cell, 19, 256-269.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.2 expression in insect cell Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.2 additional information suppression by RNAi leads to a defective cell wall consisting of a loose network of fibrils resembling the inner and outer W1 and W7-layers of the wild-type cell wall. The nine remaining P4H-like polypeptides of Chlamydomonas cannot fully compensate for the lack of isoform Cr-P4H-1 activity Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 Pyridine-2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 Pyridine-2,5-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 Zn2+
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.025
-
 poly(L-Pro) Mr 30000-40000 da, complex of recombinant subunits PHY-4.1 and PDI2 Chlamydomonas reinhardtii
1.14.11.2 0.1
-
 (L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
1.14.11.2 0.1
-
 (L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
1.14.11.2 0.1
-
 (L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 L-proline
-
 Chlamydomonas reinhardtii
1.14.11.2 0.12
-
 (L-Ser-L-Pro)19 L-proline
-
 Chlamydomonas reinhardtii
1.14.11.2 0.14
-
 poly(L-Pro) Mr 5000-10000 Da, complex of recombinant subunits PHY-4.1 and PDI2 Chlamydomonas reinhardtii
1.14.11.2 0.25
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.14.11.2 1.5
-
 (Pro-Pro-Gly)10 L-proline
-
 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ Km value 0.03 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 28315
-
 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii
1.14.11.2 29000
-
 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii
1.14.11.2 35000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Chlamydomonas reinhardtii
-
 isoform Cr-P4H-1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (L-Ser-L-Pro)19 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro)19 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Ala-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Glu-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (L-Ser-L-Pro-L-Lys-L-Pro-L-Pro)5 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 monomer 1 * 29000, SDS-PAGe, 1 * 28315, calculated for His-tagged Cr-P4H-1A without the signal peptide Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate Km value 0.02 mM Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.01
-
 Zn2+
-
 Chlamydomonas reinhardtii
1.14.11.2 0.07
-
 Pyridine-2,4-dicarboxylate
-
 Chlamydomonas reinhardtii
1.14.11.2 0.1
-
 Pyridine-2,5-dicarboxylate
-
 Chlamydomonas reinhardtii