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Literature summary extracted from

  • Catarecha, P.; Segura, M.D.; Franco-Zorrilla, J.M.; Garcia-Ponce, B.; Lanza, M.; Solano, R.; Paz-Ares, J.; Leyva, A.
    A mutant of the Arabidopsis phosphate transporter PHT1;1 displays enhanced arsenic accumulation (2007), Plant Cell, 19, 1123-1133.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.1 gene PHO84, DNA and amino acid sequence determination and analysis, expression analysis Saccharomyces cerevisiae
7.3.2.1 gene PHT1;1 mapped to locus to chromosome V, expression analysis, overview Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.1 additional information overexpression of the s(V)-tolerant missense mutant pht1;1-3 results in decreased phosphate content and enhanced arsenic accumulation, the mutant accumulates double the arsenic found in wild-type plants, the As(V) tolerance phenotypes are enhanced in the pht1;1-3 expressor lines, pht1;1-3 exhibits a constitutive phosphate starvation response, overview Arabidopsis thaliana
7.3.2.1 additional information strain PAM1 is transformed with PHO84pht1;1-3, a mutagenized version of the PHO84 cDNA, encoding a Gly-to-Glu mutation identical to the one present in the Arabidopsis thaliana pht1;1-3 allele. In the presence of 550 mM Pi, cells expressing PHO84pht1;1-3 exhibits more phosphatase activity than cells transformed with either PHO84 cDNA or the empty vector PAM1, the rate of Pi and As(V) transport in cells expressing PHO84pht1;1-3 is significantly lower than in the original PAM1, overview Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.3.2.1 arsenic acid As(V) represses the activation of genes specifically involved in Pi uptake, while inducing others transcriptionally regulated by As(V), suggesting that converse signaling pathways are involved in plant responses to As(V) and low phosphate availability, As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview Arabidopsis thaliana
7.3.2.1 arsenic acid As(V) downregulates genes transcriptionally regulated by Pi starvation, being particularly efficient in the repression of the Pi/As(V) uptake system. The repression occurs conversely to the activation of As(V)-responsive genes, overview Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.1 membrane
-
Saccharomyces cerevisiae 16020
-
7.3.2.1 membrane
-
Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.1 ATP + H2O + phosphate/out Saccharomyces cerevisiae
-
ADP + phosphate + phosphate/in
-
?
7.3.2.1 ATP + H2O + phosphate/out Arabidopsis thaliana
-
ADP + phosphate + phosphate/in
-
?
7.3.2.1 additional information Arabidopsis thaliana the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.1 Arabidopsis thaliana
-
gene pht1;1
-
7.3.2.1 Saccharomyces cerevisiae
-
strainPAM1, gene PHO84
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.3.2.1 root
-
Arabidopsis thaliana
-
7.3.2.1 root hair
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.1 ATP + H2O + phosphate/out
-
Saccharomyces cerevisiae ADP + phosphate + phosphate/in
-
?
7.3.2.1 ATP + H2O + phosphate/out
-
Arabidopsis thaliana ADP + phosphate + phosphate/in
-
?
7.3.2.1 additional information the exceptional toxicity of arsenate, As(V), is derived from its close chemical similarity to phosphate, which allows the metalloid to be easily incorporated into plant cells through the high-affinity Pi transport system Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.1 high-affinity Pi transporter
-
Saccharomyces cerevisiae
7.3.2.1 Pho84p
-
Saccharomyces cerevisiae
7.3.2.1 phosphate transporter
-
Arabidopsis thaliana
7.3.2.1 PHT1;1
-
Arabidopsis thaliana
7.3.2.1 Pi transporter
-
Saccharomyces cerevisiae