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Literature summary extracted from

  • Chua, C.S.; Biermann, D.; Goo, K.S.; Sim, T.S.
    Elucidation of active site residues of Arabidopsis thaliana flavonol synthase provides a molecular platform for engineering flavonols (2008), Phytochemistry, 69, 66-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 expression in Escherichia coli as glutathione S-transferase fusion protein Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.20.6 identification of putative residues for binding ferrous iron, i.e. H221, D223 and H277, binding 2-oxoglutarate, i.e. R287 and S289, and dihydroquercetin, i.e. H132, F134, K202, F293 and E295 via computational analyses Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.20.6 D223E no catalytic activity Arabidopsis thaliana
1.14.20.6 E295L 7% of wild-type activity Arabidopsis thaliana
1.14.20.6 E295Q 48% of wild-type activity Arabidopsis thaliana
1.14.20.6 F134A 15% of wild-type activity Arabidopsis thaliana
1.14.20.6 F134L 54% of wild-type activity Arabidopsis thaliana
1.14.20.6 F293A 8% of wild-type activity Arabidopsis thaliana
1.14.20.6 F293L 9% of wild-type activity Arabidopsis thaliana
1.14.20.6 H132F 124% of wild-type activity Arabidopsis thaliana
1.14.20.6 H132Y 83% of wild-type activity Arabidopsis thaliana
1.14.20.6 H221W no catalytic activity Arabidopsis thaliana
1.14.20.6 H277F no catalytic activity Arabidopsis thaliana
1.14.20.6 K202M 24% of wild-type activity Arabidopsis thaliana
1.14.20.6 K202R 12% of wild-type activity Arabidopsis thaliana
1.14.20.6 R287K no catalytic activity Arabidopsis thaliana
1.14.20.6 S289T 48% of wild-type activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.6 0.027
-
 dihydroquercetin mutant H132F, 37°C Arabidopsis thaliana
1.14.20.6 0.031
-
 dihydroquercetin mutant H132Y, 37°C Arabidopsis thaliana
1.14.20.6 0.059
-
 dihydroquercetin wild-type, 37°C Arabidopsis thaliana
1.14.20.6 0.07
-
 dihydroquercetin mutant E295Q, 37°C Arabidopsis thaliana
1.14.20.6 0.129
-
 dihydroquercetin mutant F134L, 37°C Arabidopsis thaliana
1.14.20.6 0.32
-
 dihydroquercetin mutant S289T, 37°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.6 38000
-
 x * 38000, SDS-PAGE, x * 64000, SDS-PAGE of fusion protein with glutathione S-transferase Arabidopsis thaliana
1.14.20.6 64000
-
 x * 38000, SDS-PAGE, x * 64000, SDS-PAGE of fusion protein with glutathione S-transferase Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Arabidopsis thaliana Q96330
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.20.6 26.9
-
 wild-type, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 dihydroquercetin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana quercetin + succinate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.20.6 ? x * 38000, SDS-PAGE, x * 64000, SDS-PAGE of fusion protein with glutathione S-transferase Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.20.6 0.015
-
 dihydroquercetin mutant E295Q, 37°C Arabidopsis thaliana
1.14.20.6 0.019
-
 dihydroquercetin mutant F134L, 37°C Arabidopsis thaliana
1.14.20.6 0.024
-
 dihydroquercetin mutant S289T, 37°C Arabidopsis thaliana
1.14.20.6 0.03
-
 dihydroquercetin mutant H132Y, 37°C Arabidopsis thaliana
1.14.20.6 0.034
-
 dihydroquercetin mutant H132F, 37°C Arabidopsis thaliana
1.14.20.6 0.056
-
 dihydroquercetin wild-type, 37°C Arabidopsis thaliana