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Literature summary extracted from

  • Grzyb, J.; Malec, P.; Rumak, I.; Garstka, M.; Strzalka, K.
    Two isoforms of ferredoxin:NADP(+) oxidoreductase from wheat leaves: purification and initial biochemical characterization (2008), Photosynth. Res., 96, 99-112.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 chloroplast
-
 Spinacia oleracea 9507
-
1.18.1.2 chloroplast
-
 Triticum aestivum 9507
-
1.18.1.2 thylakoid membrane the enzyme is associated with the stromal side of the thylakoid membrane in the chloroplast Spinacia oleracea 42651
-
1.18.1.2 thylakoid membrane the enzyme is associated with the stromal side of the thylakoid membrane in the chloroplast Triticum aestivum 42651
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 34290
-
 FNR-A, ESI mass spectrometry Triticum aestivum
1.18.1.2 34300
-
 FNR-A, gel filtration Triticum aestivum
1.18.1.2 35000
-
 FNR-A, SDS-PAGE Triticum aestivum
1.18.1.2 35440
-
 FNR-B, ESI mass spectrometry Triticum aestivum
1.18.1.2 35500
-
 FNR-B, gel filtration Triticum aestivum
1.18.1.2 36000
-
 SDS-PAGE Spinacia oleracea
1.18.1.2 36000
-
 FNR-B, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Spinacia oleracea
-
-
-
1.18.1.2 Triticum aestivum Q8RVZ8
-
-
1.18.1.2 Triticum aestivum Q8RVZ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 ammonium sulfate precipitation, gel filtration, the TSK column chromatography, and MonoQ column chromatography Triticum aestivum
1.18.1.2 ammonium sulfate precipitation. gel filtration, the TSK column chromatography, and MonoQ column chromatography Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.2 leaf
-
 Spinacia oleracea
-
1.18.1.2 leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 478.6
-
 crude extract Triticum aestivum
1.18.1.2 11110
-
 after 501.79fold purification Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 dibromothymoquinone + NADPH FNR has also diaphorase activity Spinacia oleracea reduced dibromothymoquinone + NADP+
-
 ?
1.18.1.2 dibromothymoquinone + NADPH FNR has also diaphorase activity Triticum aestivum reduced dibromothymoquinone + NADP+
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Spinacia oleracea oxidized ferredoxin + NADPH
-
 r
1.18.1.2 reduced ferredoxin + NADP+
-
 Triticum aestivum oxidized ferredoxin + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin:NADP(+) oxidoreductase
-
 Spinacia oleracea
1.18.1.2 ferredoxin:NADP(+) oxidoreductase
-
 Triticum aestivum
1.18.1.2 FNR
-
 Spinacia oleracea
1.18.1.2 FNR-A isozyme Triticum aestivum
1.18.1.2 FNR-B isozyme Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 Ferredoxin
-
 Spinacia oleracea
1.18.1.2 Ferredoxin
-
 Triticum aestivum
1.18.1.2 NADP+
-
 Spinacia oleracea
1.18.1.2 NADP+
-
 Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.18.1.2 Triticum aestivum isoelectric focusing 5.5 4.8
1.18.1.2 Triticum aestivum isoelectric focusing, isozymes FNR-A and FNR-B 5.5 4.8