EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.3 | additional information | immobilization of the FMN reductase-luciferase complex | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.3 | additional information | - |
additional information | kinetics of FMN reductase-luciferase complex formation, overview | Vibrio harveyi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.30 | 24600 | - |
2 * 24600, X-ray crystallography | Aliivibrio fischeri |
1.5.1.30 | 26300 | - |
2 * 26300, X-ray crystallography | Vibrio harveyi |
1.5.1.30 | 49200 | - |
X-ray crystallography | Aliivibrio fischeri |
1.5.1.30 | 52600 | - |
X-ray crystallography | Vibrio harveyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | RCHO + FMNH2 + O2 | Aliivibrio fischeri | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview | RCOOH + FMN + H2O + hnu | - |
? | |
1.14.14.3 | RCHO + FMNH2 + O2 | Vibrio harveyi | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview | RCOOH + FMN + H2O + hnu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.30 | Aliivibrio fischeri | - |
- |
- |
1.5.1.30 | Vibrio harveyi | - |
- |
- |
1.14.14.3 | Aliivibrio fischeri | - |
- |
- |
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.1.30 | reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | ping-pong mechanism | Aliivibrio fischeri | |
1.5.1.30 | reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | ping-pong mechanism | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.30 | FMN + NAD(P)H | - |
Aliivibrio fischeri | FMNH2 + NAD(P)+ | - |
r | |
1.5.1.30 | FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
r | |
1.5.1.30 | riboflavin + NAD(P)H | - |
Aliivibrio fischeri | reduced riboflavin + NAD(P)+ | - |
r | |
1.5.1.30 | riboflavin + NADPH | - |
Vibrio harveyi | reduced riboflavin + NADP+ | - |
r | |
1.14.14.3 | RCHO + FMNH2 + O2 | - |
Aliivibrio fischeri | RCOOH + FMN + H2O + hnu | - |
? | |
1.14.14.3 | RCHO + FMNH2 + O2 | - |
Vibrio harveyi | RCOOH + FMN + H2O + hnu | - |
? | |
1.14.14.3 | RCHO + FMNH2 + O2 | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview | Aliivibrio fischeri | RCOOH + FMN + H2O + hnu | - |
? | |
1.14.14.3 | RCHO + FMNH2 + O2 | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview | Vibrio harveyi | RCOOH + FMN + H2O + hnu | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.30 | homodimer | 2 * 24600, X-ray crystallography | Aliivibrio fischeri |
1.5.1.30 | homodimer | 2 * 26300, X-ray crystallography | Vibrio harveyi |
1.5.1.30 | monomer | in solution FRPVh undergoes a monomer-dimer equilibrium | Aliivibrio fischeri |
1.5.1.30 | monomer | in solution FRPVh undergoes a monomer-dimer equilibrium | Vibrio harveyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.30 | FRGvf | - |
Aliivibrio fischeri |
1.5.1.30 | FRPvh | - |
Vibrio harveyi |
1.14.14.3 | luciferase | - |
Aliivibrio fischeri |
1.14.14.3 | luciferase | - |
Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.30 | FMN | contains one FMN cofactor per monomer, the FMN cofactor of FRPVh can be replaced by 2-thioFMN to yield a catalytically active reconstituted reductase | Aliivibrio fischeri | |
1.5.1.30 | FMN | contains one FMN cofactor per monomer, the FMN cofactor of FRPVh can be replaced by 2-thioFMN to yield a catalytically active reconstituted reductase | Vibrio harveyi | |
1.5.1.30 | NAD(P)H | - |
Aliivibrio fischeri | |
1.5.1.30 | NADPH | - |
Vibrio harveyi | |
1.14.14.3 | FMNH2 | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, overview | Aliivibrio fischeri | |
1.14.14.3 | FMNH2 | reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, overview | Vibrio harveyi |