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Literature summary extracted from

  • Viviani, V.R.; Silva Neto, A.J.; Arnoldi, F.G.; Barbosa, J.A.; Ohmiya, Y.
    The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: a solvent gate for the active site of pH-sensitive luciferases (2007), Photochem. Photobiol., 84, 138-144.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expressed in Escherichia coli BL21(DE3) cells Phrixothrix vivianii
1.13.12.7 expressed in Escherichia coli XL1-Blue cells Cratomorphus distinctus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 F227Y the mutation causes dramatic redshift and temporal shift Cratomorphus distinctus
1.13.12.7 F227Y the mutation results in a unique time-dependent shift from red to yellow-green Macrolampis sp2
1.13.12.7 F260L the mutation results in a much less pH-sensitive enzyme displaying only a 10 nm redshift and broadening of the spectra at pH 6 Cratomorphus distinctus
1.13.12.7 G228A the mutation results in a large redshift Phrixothrix vivianii
1.13.12.7 G228A the mutation results in a large redshift Macrolampis sp2
1.13.12.7 I226A the mutation causes dramatic redshift and temporal shift Cratomorphus distinctus
1.13.12.7 L260F mutation results in a 20 nm redshift without affecting pH-insensitivity Pyrearinus termitilluminans
1.13.12.7 V227A mutant displays a small redshift Pyrearinus termitilluminans
1.13.12.7 V227F mutation results in a 60 nm redshift Pyrearinus termitilluminans
1.13.12.7 V227I mutant displays a small redshift Pyrearinus termitilluminans
1.13.12.7 V227S mutant displays a small redshift Pyrearinus termitilluminans
1.13.12.7 Y227A mutant shows enzymatic behaviour similar to the wild type enzyme Phrixothrix hirtus
1.13.12.7 Y227A the mutation causes dramatic red- and time-dependent shifts Phrixothrix vivianii
1.13.12.7 Y227A the substitution results in a weak and unstable red mutant Macrolampis sp2

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Cratomorphus distinctus Q5USC8
-
-
1.13.12.7 Macrolampis sp2
-
-
-
1.13.12.7 Phrixothrix hirtus Q9U4U7
-
-
1.13.12.7 Phrixothrix vivianii Q9U4U8
-
-
1.13.12.7 Pyrearinus termitilluminans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 agarose-Ni2+ column chromatography Phrixothrix vivianii
1.13.12.7 agarose-Ni2+ column chromatography Pyrearinus termitilluminans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-luciferin + O2 + ATP
-
 Cratomorphus distinctus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-luciferin + O2 + ATP
-
 Phrixothrix vivianii oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-luciferin + O2 + ATP
-
 Macrolampis sp2 oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-luciferin + O2 + ATP
-
 Pyrearinus termitilluminans oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?
1.13.12.7 D-luciferin + O2 + ATP
-
 Phrixothrix hirtus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 beetle luciferase
-
 Cratomorphus distinctus
1.13.12.7 beetle luciferase
-
 Phrixothrix vivianii
1.13.12.7 beetle luciferase
-
 Macrolampis sp2
1.13.12.7 beetle luciferase
-
 Pyrearinus termitilluminans
1.13.12.7 beetle luciferase
-
 Phrixothrix hirtus
1.13.12.7 firefly luciferase
-
 Cratomorphus distinctus
1.13.12.7 firefly luciferase
-
 Phrixothrix vivianii
1.13.12.7 firefly luciferase
-
 Macrolampis sp2
1.13.12.7 firefly luciferase
-
 Pyrearinus termitilluminans
1.13.12.7 firefly luciferase
-
 Phrixothrix hirtus