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Literature summary extracted from

  • Lu, J.Z.; Muench, S.P.; Allary, M.; Campbell, S.; Roberts, C.W.; Mui, E.; McLeod, R.L.; Rice, D.W.; Prigge, S.T.
    Type I and type II fatty acid biosynthesis in Eimeria tenella: enoyl reductase activity and structure (2007), Parasitology, 134, 1949-1962.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expressed in Escherichia coli BL21-Star(DE3) cells Eimeria tenella

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 triclosan potent inhibitor Eimeria tenella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.04
-
 crotonyl-CoA in 100 mM sodium phosphate (pH 7.5) Eimeria tenella
1.3.1.9 0.06
-
 NADH in 100 mM sodium phosphate (pH 7.5) Eimeria tenella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 35000
-
 SDS-PAGE Eimeria tenella

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Eimeria tenella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 HiTrap metal chelating column chromatography, HiTrap SP FastFlow column chromatography, and amylose column chromatography Eimeria tenella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Eimeria tenella butyryl-CoA + NAD+
-
 ?
1.3.1.9 trans-2-enoyl-ACP + NADH + H+
-
 Eimeria tenella acyl-ACP + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 tetramer native PAGE Eimeria tenella

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 ENR
-
 Eimeria tenella
1.3.1.9 NADH-dependent enoyl reductase
-
 Eimeria tenella

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Eimeria tenella

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.9 0.00006
-
-
 Eimeria tenella triclosan