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Literature summary extracted from

  • Singh, A.R.; Joshi, S.; Arya, R.; Kayastha, A.M.; Srivastava, K.K.; Tripathi, L.M.; Saxena, J.K.
    Molecular cloning and characterization of Brugia malayi hexokinase (2008), Parasitol. Int., 57, 354-361.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.1 glucose-6-phosphate 10 mM, recombinant enzyme is 3fold activated Brugia malayi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Escherichia coli Brugia malayi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 1,10-phenanthroline 20 mM, 96% inhibition Brugia malayi
2.7.1.1 ADP 0.0001 mM, 50% inhibition. Noncompetitive inhibition with the substrate glucose Brugia malayi
2.7.1.1 CaCl2 10 mM, 98% inhibition Brugia malayi
2.7.1.1 D-glucosamine
-
 Brugia malayi
2.7.1.1 D-glucose 6-phosphate
-
 Brugia malayi
2.7.1.1 diphosphate mixed type of inhibition when ATP is used as substrate Brugia malayi
2.7.1.1 EDTA 40 mM, 91% inhibition Brugia malayi
2.7.1.1 KCl 10 mM, 92% inhibition Brugia malayi
2.7.1.1 mannoheptulose
-
 Brugia malayi
2.7.1.1 Mg2+ 100 mM, 72% inhibition Brugia malayi
2.7.1.1 N-acetyl glucosamine
-
 Brugia malayi
2.7.1.1 NaCl 10 mM, 96% inhibition Brugia malayi
2.7.1.1 p-chloromercuribenzoate 0.05 mM, 72% inhibition Brugia malayi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.035
-
 D-glucose
-
 Brugia malayi
2.7.1.1 1.09
-
 ATP
-
 Brugia malayi
2.7.1.1 75
-
 D-fructose
-
 Brugia malayi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 72000
-
 4 * 72000, SDS-PAGE Brugia malayi

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Brugia malayi Q56VN6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 recombinant enzyme Brugia malayi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-fructose
-
 Brugia malayi ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 ATP + D-galactose
-
 Brugia malayi ?
-
 ?
2.7.1.1 ATP + D-glucose
-
 Brugia malayi ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-maltose
-
 Brugia malayi ?
-
 ?
2.7.1.1 ATP + D-mannose
-
 Brugia malayi ADP + D-mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 tetramer 4 * 72000, SDS-PAGE Brugia malayi

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 BmHk
-
 Brugia malayi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8.4
-
-
 Brugia malayi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.009
-
 diphosphate
-
 Brugia malayi
2.7.1.1 0.55
-
 ADP substrate glucose Brugia malayi