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Literature summary extracted from

  • Wierzchowski, J.; Iwanska, B.; Bzowska, A.; Shugar, D.
    Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies (2007), Nucleosides Nucleotides Nucleic Acids, 26, 849-854.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 7-ketopyrazolo[4,3-d]pyrimidine inhibits phosphorolysis of 7-methylguanosine uncompetitively, inhibits synthesis of 8-azaguanosine competitively Bos taurus
2.4.2.1 7KPP non-substrate inhibitor of both the phosphorolytic and reverse synthetic pathways, inhibits synthesis of 8-azaguanosine from 8-azaguanine competitively Bos taurus
2.4.2.1 formycin B inhibits phosphorolysis of 7-methylguanosine uncompetitively; weak, uncompetitive inhibitor. Formycin B forms a weakly fluorescent complex with the enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus
-
-
-
2.4.2.1 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methylguanosine + phosphate
-
 Bos taurus 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 8-azaguanine + alpha-D-ribose 1-phosphate
-
 Bos taurus 8-azaguanosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.002
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.3, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
2.4.2.1 0.0028 0.0035 7-ketopyrazolo[4,3-d]pyrimidine pH 6.5, concentration of the substrate 8-azaguanosine is 0.05-0.25 mM Bos taurus
2.4.2.1 0.0036
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.9, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
2.4.2.1 0.0037
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
2.4.2.1 0.0042
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
2.4.2.1 0.038
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 6.0, concentration of the substrate 7-methylguanosine is 0.032 mM Bos taurus
2.4.2.1 0.054
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM Bos taurus
2.4.2.1 0.073
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM Bos taurus
2.4.2.1 0.093
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.0, concentration of the substrate 7-methylguanosine is 0.032 mM Bos taurus
2.4.2.1 0.1
-
 formycin B inhibition of phosphorolysis of 7-methylguanosine Bos taurus
2.4.2.1 0.1
-
 formycin B phosphorolysis of 7-methylguanosine Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.1 0.002 0.004 synthesis of 8-azaguanosine from 8-azaguanine Bos taurus 7KPP
2.4.2.1 0.035 0.1 phosphorolysis of 7-methylguanosine Bos taurus 7KPP
2.4.2.1 0.098
-
 pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM Bos taurus 7-ketopyrazolo[4,3-d]pyrimidine
2.4.2.1 0.098
-
 pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM Bos taurus 7-ketopyrazolo[4,3-d]pyrimidine