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Literature summary extracted from

  • Schroeven, L.; Lammens, W.; Van Laere, A.; Van den Ende, W.
    Transforming wheat vacuolar invertase into a high affinity sucrose:sucrose 1-fructosyltransferase (2008), New Phytol., 180, 822-831.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.99 additional information transformation of invertase EC 3.2.1.26 into sucrose:sucrose 1-fructosyltransferase by disruption of hydrogen bonding network by site directed mutagenesis in the 23WMNDPNG29 motif. Invertase mutant W23Y shows moderate increase in 1-kestose production, such as invertase mutant N25S. Double mutant W23Y/N25S shows a 17fold increase in transfructosylation capacity Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.99 43
-
 sucrose invertase mutant W23Y/N25S, 30°C, pH 5.0 Triticum aestivum
2.4.1.99 215
-
 sucrose invertase mutant W23Y, 30°C, pH 5.0 Triticum aestivum
2.4.1.99 551
-
 sucrose 30°C, pH 5.0 Triticum aestivum
2.4.1.99 551
-
 sucrose wild-type sucrose:sucrose 1-fructosyltransferase, 30°C, pH 5.0 Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Triticum aestivum Q575T1
-
-
2.4.1.99 Triticum aestivum Q8W430
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 sucrose + sucrose
-
 Triticum aestivum 1-kestose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.99 sucrose:sucrose 1-fructosyltransferase
-
 Triticum aestivum