Literature summary extracted from

Chakrabarti, M.; Meekins, K.M.; Gavilano, L.B.; Siminszky, B.
Inactivation of the cytochrome P450 gene CYP82E2 by degenerative mutations was a key event in the evolution of the alkaloid profile of modern tobacco (2007), New Phytol., 175, 565-574.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.14.B11	expression in Saccharomyces cerevisiae	Nicotiana sylvestris

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.14.B11	E375K	loss of catalytic activity	Nicotiana sylvestris
1.14.14.B11	additional information	analysis of polymorphic variations of the CYP82E2 orthologs in Nicotiana sylvestris and various Nicotiana tabacum genotypes	Nicotiana sylvestris
1.14.14.B11	W422L	loss of catalytic activity	Nicotiana sylvestris

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.14.B11	0.015	-	Nicotine	pH 7.1, 23°C	Nicotiana sylvestris

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.B11	Nicotiana sylvestris	A7KMF3	isoform CYP82E2	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.14.B11	leaf	highly active in senescing leaf	Nicotiana sylvestris	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.B11	nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Nicotiana sylvestris	nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

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Release 2023.1 (January 2023)