Literature summary extracted from
Hattori, M.; Tanaka, Y.; Fukai, S.; Ishitani, R.; Nureki, O.
Crystal structure of the MgtE Mg2+ transporter (2007), Nature, 448, 1072-1075.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
7.2.2.14 |
gene mgtE, overexpression of N-terminally His-tagged full-length enzyme and cytosolic domain in Escherichia coli strain C41 (DE3) |
Thermus thermophilus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
7.2.2.14 |
selenomethionine-labeled full-length enzyme and soluble cytosolic domain in presence and absence of Mg2+, 10 mg/ml full-length protein with 0.1% dodecylmaltoside, by vapour diffusion over 32-34% 2-methyl-2,4-pentanediol, 40 mM magnesium acetate, and 100 mM MES, pH 6.0, cytosolic domain with bound Mg2+ by vapour diffusion over solutions containing 18-22% PEG 400, 0.2M MgCl2, and 0.1M HEPES, pH 7.4, or cytosolic domain in the absence of Mg2+ in 20% PEG 3350, 0.2 M ammonium acetate, and 0.5% octyl-beta-D-glucopyranoside, X-ray diffraction structure determination at 2.3-3.9 A resolution, multiple anomaleous dispersion method |
Thermus thermophilus |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
7.2.2.14 |
membrane |
the transmembrane helices are connected by 5 loops, structure, overview |
Thermus thermophilus |
16020 |
- |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
7.2.2.14 |
ATP + H2O + Mg2+/out |
Thermus thermophilus |
Mg21 homeostasis mechanism model, overview |
ADP + phosphate + Mg2+/in |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
7.2.2.14 |
Thermus thermophilus |
Q5SMG8 |
gene mgtE |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
7.2.2.14 |
recombinant N-terminally His-tagged cytosolic domain by nickel affinity chromatography, tag cleavage by thrombin, anion exchange chromatography and gel filtration, recombinant N-terminally His-tagged full-length MgtE from Escherichia coli strain C41 (DE3) by nickel affinity chromatography and gel filtration |
Thermus thermophilus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
7.2.2.14 |
ATP + H2O + Mg2+/out |
Mg21 homeostasis mechanism model, overview |
Thermus thermophilus |
ADP + phosphate + Mg2+/in |
- |
? |
|
7.2.2.14 |
ATP + H2O + Mg2+/out |
transport of the fully hydrated Mg2+ ion, one Mg2+ is bound to conserved Asp432 within the solvent-accessible pore of the transmembrane segement, four Mg2+ ions are bound at the interface between the connecting helices and the other domains, Mg2+ binding structure, overview |
Thermus thermophilus |
ADP + phosphate + Mg2+/in |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
7.2.2.14 |
Mg2+ transporter |
- |
Thermus thermophilus |
7.2.2.14 |
MgtE |
- |
Thermus thermophilus |