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Literature summary extracted from

  • Ishikawa, T.; Okazaki, K.; Kuroda, H.; Itoh, K.; Mitsui, T.; Hori, H.
    Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development (2007), Mol. Plant Pathol., 8, 623-637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli Brassica rapa
3.5.5.4 expressed in Escherichia coli Brassica rapa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 0.23
-
 3-Phenylpropionitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 0.34
-
 4-phenylbutyronitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 0.35
-
 heptanenitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 2.12
-
 indole-3-acetonitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 2.49
-
 allyl cyanide isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.4 0.69
-
 3-Cyano-L-alanine in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Brassica rapa A3QYW3 NIT-T2; cultivar Natsumaki
-
3.5.5.4 Brassica rapa
-
 cultivar Natsumaki
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration Brassica rapa
3.5.5.4 Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration Brassica rapa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 root
-
 Brassica rapa
-
3.5.5.4 root
-
 Brassica rapa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 3-phenylpropionitrile + H2O
-
 Brassica rapa 3-phenylpropionic acid + NH3
-
 ?
3.5.5.1 4-phenylbutyronitrile + H2O
-
 Brassica rapa 4-phenylbutyric acid + NH3
-
 ?
3.5.5.1 allyl cyanide + H2O
-
 Brassica rapa prop-2-enoic acid + NH3
-
 ?
3.5.5.1 heptanenitrile + H2O
-
 Brassica rapa heptanoic acid + NH3
-
 ?
3.5.5.1 indole-3-acetonitrile + H2O poor substrate Brassica rapa indole-3-acetic acid + NH3
-
 ?
3.5.5.1 additional information 3-cyano-L-alanine is no substrate Brassica rapa ?
-
 ?
3.5.5.4 3-cyano-L-alanine + H2O
-
 Brassica rapa L-aspartate + NH3
-
 ?
3.5.5.4 additional information indole-3-acetonitrile is no substrate for NIT-T4 Brassica rapa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NIT-T2 isozyme Brassica rapa
3.5.5.4 NIT-T4
-
 Brassica rapa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.1 0.29
-
 indole-3-acetonitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 15.8
-
 4-phenylbutyronitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 19.8
-
 allyl cyanide isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 40.8
-
 heptanenitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.1 79.7
-
 3-Phenylpropionitrile isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa
3.5.5.4 19.4
-
 3-Cyano-L-alanine in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C Brassica rapa