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Literature summary extracted from

  • Zarnowski, R.; Cooper, K.G.; Brunold, L.S.; Calaycay, J.; Woods, J.P.
    Histoplasma capsulatum secreted gamma-glutamyltransferase reduces iron by generating an efficient ferric reductant (2008), Mol. Microbiol., 70, 352-368.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 the enzyme is overexpressed under the control of a constitutive Histoplasma capsulatum-native promoter Histoplasma capsulatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.75
-
 5-L-glutamyl-4-nitroanilide hydrolysis Histoplasma capsulatum
2.3.2.2 0.84
-
 5-L-glutamyl-4-nitroanilide reaction in the presence of 20 mM glycylglycine Histoplasma capsulatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 37000
-
 x * 37000 + x * 50000, SDS-PAGE Histoplasma capsulatum
2.3.2.2 50000
-
 x * 37000 + x * 50000, SDS-PAGE Histoplasma capsulatum
2.3.2.2 320000
-
 gel filtration Histoplasma capsulatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 glutathione + amino acid Histoplasma capsulatum Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Histoplasma capsulatum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein Ggt1 occurrs as a very large glycoprotein complex Histoplasma capsulatum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Histoplasma capsulatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Histoplasma capsulatum 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + H2O
-
 Histoplasma capsulatum L-glutamate + 4-nitroaniline
-
 ?
2.3.2.2 glutathione + amino acid Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range Histoplasma capsulatum L-cysteinylglycine + 5-L-glutamyl-amino acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 ? x * 37000 + x * 50000, SDS-PAGE Histoplasma capsulatum

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 GGT1
-
 Histoplasma capsulatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.2.2 Histoplasma capsulatum chromatofocusing
-
 4.8