EC Number | Cloned (Comment) | Organism |
---|---|---|
7.4.2.1 | - |
Anabaena sp. |
7.4.2.2 | ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA. Structure of the strain PCC 7120 genomic regions carrying the bgtB gene and the natF-natG-natH-bgtA gene cluster, overview, expression of the Bgt and N-II transporters as GFP-tagged proteins in strain PCC 7120 | Anabaena sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.4.2.2 | additional information | construction of deletion mutants of ORFs encoding BgtA, BgtB, NatF, NatG, and NatH, all showing altered substrate specificity compared to the wild-type enzyme, amino acid release and diazotrophy in the mutants, overview | Anabaena sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.4.2.1 | membrane | - |
Anabaena sp. | 16020 | - |
7.4.2.2 | membrane | - |
Anabaena sp. | 16020 | - |
7.4.2.2 | additional information | cellular and subcellular location of the Bgt and N-II transporters, in nitrate-grown filaments, the recombinant GFP-tagged proteins are observed in all the cells of the filament, with a somewhat higher signal in the periphery than in the centre of the cells and, especially, in the intercellular septa, overview | Anabaena sp. | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.1 | ATP + H2O + amino acid/out | Anabaena sp. | acidic and neutral polar amino acid uptake, essential for diazotropic physiology of the bacterium, transports Asp, Glu, Asn, Gln, Met, Thr, Ala, Ser, Gly, and His | ADP + phosphate + amino acid/in | - |
? | |
7.4.2.1 | ATP + H2O + amino acid/out | Anabaena sp. | essential for basic amino acid uptake, transports Lys, Arg, Orn, His, and Gln | ADP + phosphate + amino acid/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Arg/out | Anabaena sp. | - |
ADP + phosphate + L-Arg/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Asp/out | Anabaena sp. | - |
ADP + phosphate + L-Asp/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Gln/out | Anabaena sp. | - |
ADP + phosphate + L-Gln/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Glu/out | Anabaena sp. | - |
ADP + phosphate + L-Glu/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Lys/out | Anabaena sp. | - |
ADP + phosphate + L-Lys/in | - |
? | |
7.4.2.2 | ATP + H2O + alanine/out | Anabaena sp. | high activity | ADP + phosphate + alanine/in | - |
? | |
7.4.2.2 | ATP + H2O + arginine/out | Anabaena sp. | - |
ADP + phosphate + arginine/in | - |
? | |
7.4.2.2 | ATP + H2O + glutamine/out | Anabaena sp. | - |
ADP + phosphate + glutamine/in | - |
? | |
7.4.2.2 | ATP + H2O + glycine/out | Anabaena sp. | high activity | ADP + phosphate + glycine/in | - |
? | |
7.4.2.2 | ATP + H2O + histidine/out | Anabaena sp. | - |
ADP + phosphate + histidine/in | - |
? | |
7.4.2.2 | ATP + H2O + leucine/out | Anabaena sp. | - |
ADP + phosphate + leucine/in | - |
? | |
7.4.2.2 | ATP + H2O + lysine/out | Anabaena sp. | - |
ADP + phosphate + lysine/in | - |
? | |
7.4.2.2 | ATP + H2O + nonpolar amino acid/out | Anabaena sp. | whereas Bgt is dispensable for diazotrophic growth, N-II appears to contribute together with N-I to the diazotrophic physiology of this cyanobacterium | ADP + phosphate + nonpolar amino acid/in | - |
? | |
7.4.2.2 | ATP + H2O + phenylalanine/out | Anabaena sp. | - |
ADP + phosphate + phenylalanine/in | - |
? | |
7.4.2.2 | ATP + H2O + proline/out | Anabaena sp. | - |
ADP + phosphate + proline/in | - |
? | |
7.4.2.2 | ATP + H2O + serine/out | Anabaena sp. | best substrate | ADP + phosphate + serine/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.4.2.1 | Anabaena sp. | - |
strain PCC 7120 and different bgtA, bgtB, natF, natG, and natH knockout mutants | - |
7.4.2.2 | Anabaena sp. | - |
strain PCC 7120, ORFS salr4167 and alr3187 encoding BgtA and BgtB, and ORFs alr4167, alr4164, alr4165, and alr4166 encoding NatF, NatG, and NatH respectively | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
7.4.2.1 | heterocyst | - |
Anabaena sp. | - |
7.4.2.1 | vegetative cell | - |
Anabaena sp. | - |
7.4.2.2 | heterocyst | - |
Anabaena sp. | - |
7.4.2.2 | additional information | cellular and subcellular location of the Bgt and N-II transporters, N-I that is expressed only in vegetative cells, while the Bgt and N-II systems are present in both vegetative cells and heterocysts, overview | Anabaena sp. | - |
7.4.2.2 | vegetative cell | - |
Anabaena sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.1 | ATP + H2O + amino acid/out | acidic and neutral polar amino acid uptake, essential for diazotropic physiology of the bacterium, transports Asp, Glu, Asn, Gln, Met, Thr, Ala, Ser, Gly, and His | Anabaena sp. | ADP + phosphate + amino acid/in | - |
? | |
7.4.2.1 | ATP + H2O + amino acid/out | essential for basic amino acid uptake, transports Lys, Arg, Orn, His, and Gln | Anabaena sp. | ADP + phosphate + amino acid/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Arg/out | - |
Anabaena sp. | ADP + phosphate + L-Arg/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Asp/out | - |
Anabaena sp. | ADP + phosphate + L-Asp/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Gln/out | - |
Anabaena sp. | ADP + phosphate + L-Gln/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Glu/out | - |
Anabaena sp. | ADP + phosphate + L-Glu/in | - |
? | |
7.4.2.1 | ATP + H2O + L-Lys/out | - |
Anabaena sp. | ADP + phosphate + L-Lys/in | - |
? | |
7.4.2.2 | ATP + H2O + alanine/out | high activity | Anabaena sp. | ADP + phosphate + alanine/in | - |
? | |
7.4.2.2 | ATP + H2O + arginine/out | - |
Anabaena sp. | ADP + phosphate + arginine/in | - |
? | |
7.4.2.2 | ATP + H2O + aspartate/out | low activity | Anabaena sp. | ADP + phosphate + aspartate/in | - |
? | |
7.4.2.2 | ATP + H2O + glutamate/out | low activity | Anabaena sp. | ADP + phosphate + glutamate/in | - |
? | |
7.4.2.2 | ATP + H2O + glutamine/out | - |
Anabaena sp. | ADP + phosphate + glutamine/in | - |
? | |
7.4.2.2 | ATP + H2O + glycine/out | high activity | Anabaena sp. | ADP + phosphate + glycine/in | - |
? | |
7.4.2.2 | ATP + H2O + histidine/out | - |
Anabaena sp. | ADP + phosphate + histidine/in | - |
? | |
7.4.2.2 | ATP + H2O + leucine/out | - |
Anabaena sp. | ADP + phosphate + leucine/in | - |
? | |
7.4.2.2 | ATP + H2O + lysine/out | - |
Anabaena sp. | ADP + phosphate + lysine/in | - |
? | |
7.4.2.2 | ATP + H2O + nonpolar amino acid/out | - |
Anabaena sp. | ADP + phosphate + nonpolar amino acid/in | - |
? | |
7.4.2.2 | ATP + H2O + nonpolar amino acid/out | whereas Bgt is dispensable for diazotrophic growth, N-II appears to contribute together with N-I to the diazotrophic physiology of this cyanobacterium | Anabaena sp. | ADP + phosphate + nonpolar amino acid/in | - |
? | |
7.4.2.2 | ATP + H2O + phenylalanine/out | - |
Anabaena sp. | ADP + phosphate + phenylalanine/in | - |
? | |
7.4.2.2 | ATP + H2O + proline/out | - |
Anabaena sp. | ADP + phosphate + proline/in | - |
? | |
7.4.2.2 | ATP + H2O + serine/out | best substrate | Anabaena sp. | ADP + phosphate + serine/in | - |
? | |
7.4.2.2 | additional information | substrate specificity of wild-type and mutant strains, overview | Anabaena sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.4.2.1 | More | ABC-type amino acid uptake transporter, composed of the ATPase subunit BgtA and a composite protein bearing periplasmic substrate-binding and transmembrane domains BgtB | Anabaena sp. |
7.4.2.1 | More | ABC-type amino acid uptake transporter, composed of the ATPase subunit BgtA and the NatF, NatG, and NatH proteins | Anabaena sp. |
7.4.2.2 | More | ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA | Anabaena sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.4.2.2 | ABC-type high-affinity basic amino acid uptake transporter | - |
Anabaena sp. |
7.4.2.2 | ATPase | - |
Anabaena sp. |
7.4.2.2 | BGT | - |
Anabaena sp. |