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Literature summary extracted from
- Mutation of a key residue in the type II secretion system ATPase uncouples ATP hydrolysis from protein translocation (2007), Mol. Microbiol., 65, 401-412.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.4.2.8 | using a pET29a based plasmid and pET15b for overexpressing of the protein in Escherichia coli BL21DE3 pLysS cells | Xanthomonas campestris |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.4.2.8 | R286A | XpsE mutant hydrolysis ATP at a rate five times that of the wild-type XpsE, but is non-functional in protein secretion via T2SS | Xanthomonas campestris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.4.2.8 | 0.0726 | - |
ATP | XpsE mutant R286A | Xanthomonas campestris |  |
| 7.4.2.8 | 0.0891 | - |
ATP | XpsE wild-type, full-lenght | Xanthomonas campestris | |
| 7.4.2.8 | 0.1021 | - |
ATP | XpsE, N-domain removed | Xanthomonas campestris | |
| 7.4.2.8 | 0.1061 | - |
ATP | XpsE wild-type + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
| 7.4.2.8 | 0.1198 | - |
ATP | XpsE mutant R286A, N-domain removed | Xanthomonas campestris | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.8 | ATP + H2O | Xanthomonas campestris | - |
ADP + phosphate | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.4.2.8 | Xanthomonas campestris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.4.2.8 | XpsE-Strep and its variants are purified from the Strep-Tactin Sepharose column, the XpsE with His6-tag is purified by eluting from nickel nitrilotriacetate resin | Xanthomonas campestris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.8 | ATP + H2O | - |
Xanthomonas campestris | ADP + phosphate | - |
ir | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.4.2.8 | T2SS ATPase | - |
Xanthomonas campestris |
| 7.4.2.8 | type II secretion system ATPase | - |
Xanthomonas campestris |
| 7.4.2.8 | XpsE | - |
Xanthomonas campestris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.8 | 28 | - |
ATPase assay | Xanthomonas campestris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.4.2.8 | 0.00062 | - |
ATP | XpsE wild-type, full-lenght | Xanthomonas campestris | |
| 7.4.2.8 | 0.00275 | - |
ATP | XpsE mutant R286A | Xanthomonas campestris | |
| 7.4.2.8 | 0.00305 | - |
ATP | XpsE, N-domain removed | Xanthomonas campestris | |
| 7.4.2.8 | 0.0036 | - |
ATP | XpsE mutant R286A, N-domain removed | Xanthomonas campestris | |
| 7.4.2.8 | 0.005 | - |
ATP | XpsE mutant R286A + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
| 7.4.2.8 | 0.00505 | - |
ATP | XpsE wild-type + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
| 7.4.2.8 | 0.00525 | - |
ATP | XpsE, N-domain removed + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
| 7.4.2.8 | 0.00558 | - |
ATP | XpsE mutant R286A, N-domain removed + MBP-tagged XpsL, residues 1-215 | Xanthomonas campestris | |
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