Any feedback?
Literature summary extracted from
- Bacterial homologs of eukaryotic membrane proteins: the 2-TM-GxN family of Mg2+ transporters (Review) (2007), Mol. Membr. Biol., 24, 351-356.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 7.2.2.14 | crystal structure analysis from PDB ID BBJ2 at 3.9 A resolution for the whole enzyme, and at 1.8 A resolution for the soluble domain, overview | Thermotoga maritima |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.2.2.14 | additional information | Alr1p mutants show 25-30% reduced Mg2+ content but fail to accumulate Mg2+ | Saccharomyces cerevisiae |
| 7.2.2.14 | additional information | mutation of residues near L294/M291 alters the transport properties of CorA | Thermotoga maritima |
| 7.2.2.14 | additional information | selection of Mg2+ transport defect corA mutants by screening for Co2+ resistant strains, since accumulation of Co2+ is tosic for the cells | Escherichia coli |
| 7.2.2.14 | additional information | the mgtAmgtCB mutant strain is dependent on CorA for Mg2+ uptake and is completely growth-inhibited by cation hexaamines, Mrs2p mutants can be complemented by bacterial CorA, mrs2 mutants decreases Mg2+ uptake and Mg2+ content in the mitochondria by 62% | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.14 | Al3+ | inhibits Alr1p and Alr2p; inhibits Alr1p and Alr2p | Saccharomyces cerevisiae |  |
| 7.2.2.14 | Co(III) hexaamines | inhibit Mrs2p and CorA, inhibition of Ni2+ uptake by CorA | Saccharomyces cerevisiae | |
| 7.2.2.14 | Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | Mn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | additional information | no inhibition of Mg2+ or Ni2+ uptake by CorA through Fe2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | Ni2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | ruthenium (III) hexaamines | inhibit Mrs2p and CorA, inhibition of Ni2+ uptake by CorA | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.2.2.14 | 0.015 | - |
Mg2+ | 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.2.2.14 | 0.015 | - |
Mg2+ | 20°C | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.14 | mitochondrial inner membrane | Mrs2p | Saccharomyces cerevisiae | 5743 | - |
| 7.2.2.14 | plasma membrane | Alr1p | Saccharomyces cerevisiae | 5886 | - |
| 7.2.2.14 | plasma membrane | Alr2p | Saccharomyces cerevisiae | 5886 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.14 | ATP + H2O + Mg2+/out | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | Thermotoga maritima | - |
ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | - |
ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | at high Mg2+ levels Alr1p is rapidly degraded by the ubiquitin dependent pathway | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | Mg2+ uptake by Mrs2p is functionally regulated in the mitochondria | ADP + phosphate + Mg2+/in | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.14 | Escherichia coli | - |
gene corA | - |
| 7.2.2.14 | Saccharomyces cerevisiae | P43553 | Alr2p; gene alr2 | - |
| 7.2.2.14 | Saccharomyces cerevisiae | Q02783 | Mrs2p; gene mrs2 | - |
| 7.2.2.14 | Saccharomyces cerevisiae | Q08269 | Alr1p; gene alr1 | - |
| 7.2.2.14 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene corA | - |
| 7.2.2.14 | Thermotoga maritima | Q9WZ31 | gene corA | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 7.2.2.14 | ATP + H2O + Mg2+[side 1] = ADP + phosphate + Mg2+[side 2] | Mg2+ transport mechanism, structure-function relationship, overview | Thermotoga maritima |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.2.2.14 | additional information | nonrepressible constitutive expression of CorA | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.14 | ATP + H2O + Co2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Co2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Co2+/out | - |
Escherichia coli | ADP + phosphate + Co2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | - |
Escherichia coli | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | - |
Thermotoga maritima | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | - |
Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | at high Mg2+ levels Alr1p is rapidly degraded by the ubiquitin dependent pathway | Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Mg2+/out | Mg2+ uptake by Mrs2p is functionally regulated in the mitochondria | Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| 7.2.2.14 | ATP + H2O + Ni2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Ni2+/in | - |
? | |
| 7.2.2.14 | additional information | no transport of Fe2+, Mn2+, or Ca2+ | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.14 | More | secondary structure determination and analysis | Thermotoga maritima |
| 7.2.2.14 | oligomer | Alr1p is at least a tetramer, Alr1p and Alr2p form homooligomers, but heteroligomers when overexpressed, the oligomerization of Alr1p results in a dominant negative action on the protein | Saccharomyces cerevisiae |
| 7.2.2.14 | oligomer | Alr2p forms homooligomers, but heteroligomers when overexpressed | Saccharomyces cerevisiae |
| 7.2.2.14 | pentamer | CorA | Escherichia coli |
| 7.2.2.14 | pentamer | CorA | Thermotoga maritima |
| 7.2.2.14 | pentamer | CorA | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.2.14 | ALR1 | - |
Saccharomyces cerevisiae |
| 7.2.2.14 | ALR2 | - |
Saccharomyces cerevisiae |
| 7.2.2.14 | CorA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 7.2.2.14 | CorA | - |
Escherichia coli |
| 7.2.2.14 | CorA | - |
Thermotoga maritima |
| 7.2.2.14 | More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Escherichia coli |
| 7.2.2.14 | More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Thermotoga maritima |
| 7.2.2.14 | More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Saccharomyces cerevisiae |
| 7.2.2.14 | Mrs2p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.14 | 20 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 7.2.2.14 | 20 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
