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Literature summary extracted from

  • Fice, D.; Shen, Z.; Byers, D.M.
    Purification and characterization of fatty acyl-acyl carrier protein synthetase from Vibrio harveyi (1993), J. Bacteriol., 175, 1865-1870.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.20 iodoacetamide stimulates Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.20 NEM acyl-carrier protein or ATP protects Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.20 0.02
-
 palmitoleic acid E. coli [acyl-carrier protein] Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.20 62000
-
 x * 62000, SDS-PAGE Vibrio harveyi
6.2.1.20 80000
-
 gel filtration under reducing conditions Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Vibrio harveyi
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.20 4.6
-
-
 Vibrio harveyi

Storage Stability

EC Number Storage Stability Organism
6.2.1.20 -20°C, 0.002% Triton X-100, 10% glycerol, 50% loss of activity within 1 month Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + myristic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + myristoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + palmitoleic acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + palmitoleoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 dATP + a fatty acid + [acyl-carrier protein] 25% of the activity relative to ATP Vibrio harveyi dAMP + diphosphate + fatty acid-[acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.20 ? x * 62000, SDS-PAGE Vibrio harveyi