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Literature summary extracted from

  • Kim, B.G.; Kim, J.H.; Kim, J.; Lee, C.; Ahn, J.H.
    Accumulation of flavonols in response to ultraviolet-B irradiation in soybean is related to induction of flavanone 3-beta-hydroxylase and flavonol synthase (2008), Mol. Cells, 25, 247-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 expression in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 0.087
-
 eriodictyol pH 8.0, 37°C Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Iron
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.9 42700
-
 x * 42700, calculated, x * 68000, SDS-PAGE of fusion protein with glutathione S-transferase Glycine max
1.14.11.9 68000
-
 x * 42700, calculated, x * 68000, SDS-PAGE of fusion protein with glutathione S-transferase Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.6 additional information Glycine max flavonol synthase and flavanone 3beta-hydroxylase are key enzymes in the biosynthesis of the UV-B protectant, kaempferol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Glycine max
-
 expression is induced by ultraviolet-B irradiation
-
1.14.20.6 Glycine max Q0EDG6 expression is induced by UV-B irradiation and subsequently stimulates accumulation of kaempferol glycones
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.6 leaf
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 (2S)-naringenin + 2-oxoglutarate + O2 90% of the activity with eriodictyol Glycine max (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 3'-methoxy eriodictyol + 2-oxoglutarate + O2 16% of the activity with eriodictyol Glycine max ? + succinate + CO2
-
 ?
1.14.11.9 4'-methoxy eriodictyol + 2-oxoglutarate + O2 56% of the activity with eriodictyol Glycine max ? + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2
-
 Glycine max (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
1.14.20.6 additional information flavonol synthase and flavanone 3beta-hydroxylase are key enzymes in the biosynthesis of the UV-B protectant, kaempferol Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 ? x * 42700, calculated, x * 68000, SDS-PAGE of fusion protein with glutathione S-transferase Glycine max