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Literature summary extracted from

  • Sheoran, A.; King, A.; Velasco, A.; Pero, J.M.; Garneau-Tsodikova, S.
    Characterization of TioF, a tryptophan 2,3-dioxygenase involved in 3-hydroxyquinaldic acid formation during thiocoraline biosynthesis (2008), Mol. Biosyst., 4, 622-628.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.44
-
D-tryptophan
-
Micromonospora sp.
1.13.11.52 0.65
-
L-tryptophan
-
Micromonospora sp.
1.13.11.52 2.56
-
serotonin
-
Micromonospora sp.
1.13.11.52 30.27
-
indole
-
Micromonospora sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 L-tryptophan + O2 Micromonospora sp. first step during biosynthesis of 3-hydroxyquinaldic acid N-formyl-L-kynurenine
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Micromonospora sp.
-
recombinantly expressed in Escherichia coli as a N-terminally His6-tagged protein
-
1.13.11.52 Micromonospora sp. ML1
-
recombinantly expressed in Escherichia coli as a N-terminally His6-tagged protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52
-
Micromonospora sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
Micromonospora sp. N-formyl-D-kynurenine
-
ir
1.13.11.52 D-tryptophan + O2
-
Micromonospora sp. ML1 N-formyl-D-kynurenine
-
ir
1.13.11.52 indole + O2
-
Micromonospora sp. ?
-
?
1.13.11.52 indole + O2
-
Micromonospora sp. ML1 ?
-
?
1.13.11.52 L-tryptophan + O2
-
Micromonospora sp. N-formyl-L-kynurenine
-
ir
1.13.11.52 L-tryptophan + O2 first step during biosynthesis of 3-hydroxyquinaldic acid Micromonospora sp. N-formyl-L-kynurenine
-
ir
1.13.11.52 L-tryptophan + O2
-
Micromonospora sp. ML1 N-formyl-L-kynurenine
-
ir
1.13.11.52 additional information TioF is completely inactive towards tryptamine, 5-hydroxy-L-Trp, and indole propionic acid Micromonospora sp. ?
-
?
1.13.11.52 additional information TioF is completely inactive towards tryptamine, 5-hydroxy-L-Trp, and indole propionic acid Micromonospora sp. ML1 ?
-
?
1.13.11.52 serotonin + O2
-
Micromonospora sp. ?
-
?
1.13.11.52 serotonin + O2
-
Micromonospora sp. ML1 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 TioF
-
Micromonospora sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.52 0.0068
-
D-tryptophan
-
Micromonospora sp.
1.13.11.52 0.028
-
serotonin
-
Micromonospora sp.
1.13.11.52 0.045
-
L-tryptophan
-
Micromonospora sp.
1.13.11.52 0.248
-
indole
-
Micromonospora sp.