Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guo, B.; Kai, G.; Gong, Y.; Jin, H.; Wang, Y.; Miao, Z.; Sun, X.; Tang, K.
    Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media (2007), Mol. Biol. Rep., 34, 89-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.167 ammonium ceric(IV) sulfate
-
 Taxus x media
2.3.1.167 methyl jasmonate
-
 Taxus x media
2.3.1.167 Silver nitrate
-
 Taxus x media

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.167 the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells Taxus x media

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.167 16000
-
 tag-protein Taxus x media
2.3.1.167 48980
-
 predicted molecular mass Taxus x media
2.3.1.167 65000
-
 determined by SDS-PAGE, TmDBAT plus fused tag Taxus x media

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III Taxus x media
-
 CoA + baccatin III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.167 Taxus x media Q6SQJ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.167 by affinity chromatography in Ni-nitrilotriacetic acid columns, the tag is removed by digestion with enterokinase Taxus x media

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.167 leaf
-
 Taxus x media
-
2.3.1.167 stem
-
 Taxus x media
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus x media CoA + baccatin III
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.167 10-deacetylbaccatin III-10-O-acetyl transferase
-
 Taxus x media
2.3.1.167 TmDBAT
-
 Taxus x media

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.167 Taxus x media predicted pI-value
-
 6.05