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Literature summary extracted from
- Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination (2006), Mol. Biochem. Parasitol., 150, 118-121.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.1 | expression of the chimeric mutant cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum, negative selection with 5-fluorocytosine | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | additional information | construction of a chimeric bifuntional enzyme comprising cytosine deaminase and uracil phosphoribosyl transferase activities, overview | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | 5-fluorocytosine + H2O | reaction of cytosine deaminase, the uracil phosphoribosyl transferase part in a chimeric bifunctional enzyme converts 5-fluorouracil into 5-fluoro-UMP, which is a precursor for the cell toxic 5-fluoro-UTP, used for negative selection of transgenic Plasmodium falciparum parasites | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.4.1 | cytosine deaminase | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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