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Literature summary extracted from

  • Rascon, A.; Viloria, M.E.; De-Chiara, L.; Dubra, M.E.
    Characterization of cyclic AMP phosphodiesterases in Leishmania mexicana and purification of a soluble form (2000), Mol. Biochem. Parasitol., 106, 283-292.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.53 1,10-phenanthroline 0.3 mM, more than 95% inhibition Leishmania mexicana
3.1.4.53 3-isobutyl-1-methyl-xanthine
-
 Leishmania mexicana
3.1.4.53 Ca2+
-
 Leishmania mexicana
3.1.4.53 Caffeine 50 mM, 30% inhibition of soluble enzyme Leishmania mexicana
3.1.4.53 Cibacron blue
-
 Leishmania mexicana
3.1.4.53 EDTA 0.3 mM, more than 95% inhibition Leishmania mexicana
3.1.4.53 OPC-3911
-
 Leishmania mexicana
3.1.4.53 rolipram
-
 Leishmania mexicana
3.1.4.53 theophylline
-
 Leishmania mexicana
3.1.4.53 zaprinast
-
 Leishmania mexicana
3.1.4.53 Zn2+
-
 Leishmania mexicana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.53 0.277
-
 adenosine 3',5'-cyclic phosphate 30°C Leishmania mexicana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.53 cytosol
-
 Leishmania mexicana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.53 Mg2+ stimulates Leishmania mexicana
3.1.4.53 Mn2+ stimulates Leishmania mexicana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.53 61000
-
 x * 61000, SDS-PAGE Leishmania mexicana
3.1.4.53 200000
-
 gel filtration Leishmania mexicana

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.53 Leishmania mexicana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.53
-
 Leishmania mexicana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.53 promastigote
-
 Leishmania mexicana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.53 0.02689
-
-
 Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.53 adenosine 3',5'-cyclic phosphate + H2O the enzyme is highly specific for cAMP Leishmania mexicana adenosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.53 ? x * 61000, SDS-PAGE Leishmania mexicana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.53 30
-
-
 Leishmania mexicana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.53 7.2
-
-
 Leishmania mexicana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.53 6.3 9.2 pH 6.3: about 40% of maximal activity, pH 9.2: about 80% of maximal activity Leishmania mexicana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.53 0.008
-
 soluble enzyme Leishmania mexicana Cibacron blue
3.1.4.53 0.02
-
 particulate enzyme Leishmania mexicana Cibacron blue
3.1.4.53 0.04
-
 particulate enzyme Leishmania mexicana zaprinast
3.1.4.53 0.3
-
 soluble enzyme Leishmania mexicana OPC-3911
3.1.4.53 0.6
-
 particulate enzyme Leishmania mexicana rolipram
3.1.4.53 0.7
-
 soluble enzyme Leishmania mexicana rolipram
3.1.4.53 0.8
-
 particulate enzyme Leishmania mexicana OPC-3911
3.1.4.53 2
-
 soluble enzyme Leishmania mexicana 3-isobutyl-1-methyl-xanthine
3.1.4.53 2.8
-
 soluble enzyme Leishmania mexicana zaprinast
3.1.4.53 3
-
 particulate enzyme Leishmania mexicana theophylline
3.1.4.53 3
-
 particulate enzyme Leishmania mexicana 3-isobutyl-1-methyl-xanthine
3.1.4.53 19
-
 particulate enzyme Leishmania mexicana Caffeine
3.1.4.53 30
-
 soluble enzyme Leishmania mexicana theophylline