EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.5.2 | into pET21a, His-tagged version overexpressed in Escherichia coli BL21 Origami competent cells | Helicobacter hepaticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.5.2 | additional information | mdaB mutant can be grown in microaerophilic conditions. The mutant is more sensitive to oxidative stress reagents such as H2O2, cumene hydroperoxide, t-butyl hydroperoxide, and paraquat. Protein (migration mass of about 26 kDa) is significantly upexpressed in the mutant compared to the wild-type | Helicobacter hepaticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.5.2 | Helicobacter hepaticus | - |
strain ATCC51449 | - |
1.6.5.2 | Helicobacter hepaticus ATCC 51449 | - |
strain ATCC51449 | - |
1.6.5.10 | Helicobacter hepaticus | - |
strain ATCC51449 | - |
1.6.5.10 | Helicobacter hepaticus ATCC 51449 | - |
strain ATCC51449 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.5.10 | - |
Helicobacter hepaticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.5.2 | additional information | - |
quinone or quinone-like compounds reduction assay with a specific activity ranging from 0.92-4.6 U/mg of protein | Helicobacter hepaticus |
1.6.5.2 | 4 | - |
menadione-cytochrome c reduction assay | Helicobacter hepaticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.5.2 | 1,4-naphthoquinone + NAD(P)H | - |
Helicobacter hepaticus | 1,4-naphthohydroquinone + NAD(P)+ | - |
? | |
1.6.5.2 | 1,4-naphthoquinone + NAD(P)H | - |
Helicobacter hepaticus ATCC 51449 | 1,4-naphthohydroquinone + NAD(P)+ | - |
? | |
1.6.5.2 | 2 ferricyanide + NAD(P)H | - |
Helicobacter hepaticus | 2 ferrocyanide + NAD(P)+ + H+ | - |
? | |
1.6.5.2 | 2 ferricyanide + NAD(P)H | - |
Helicobacter hepaticus ATCC 51449 | 2 ferrocyanide + NAD(P)+ + H+ | - |
? | |
1.6.5.2 | coenzyme Q0 + NAD(P)H | - |
Helicobacter hepaticus | reduced coenzyme Q0 + NADP+ | - |
? | |
1.6.5.2 | coenzyme Q0 + NAD(P)H | - |
Helicobacter hepaticus ATCC 51449 | reduced coenzyme Q0 + NADP+ | - |
? | |
1.6.5.2 | coenzyme Q1 + NAD(P)H | - |
Helicobacter hepaticus | reduced coenzyme Q1 + NADP+ | - |
? | |
1.6.5.2 | coenzyme Q1 + NAD(P)H | - |
Helicobacter hepaticus ATCC 51449 | reduced coenzyme Q1 + NADP+ | - |
? | |
1.6.5.2 | menadione + NAD(P)H | - |
Helicobacter hepaticus | reduced menadione + NADP+ | - |
? | |
1.6.5.2 | NAD(P)H + H+ + quinone | - |
Helicobacter hepaticus | NAD(P)+ + hydroquinone | - |
? | |
1.6.5.2 | oxidized dichlorophenol-indophenol + NAD(P)H | - |
Helicobacter hepaticus | reduced dichlorophenol-indophenol + NADP+ | - |
? | |
1.6.5.2 | oxidized dichlorophenol-indophenol + NAD(P)H | - |
Helicobacter hepaticus ATCC 51449 | reduced dichlorophenol-indophenol + NADP+ | - |
? | |
1.6.5.10 | 1,4-naphthoquinone + NADPH + H+ | two-electron transfer mechanism from NADPH to quinone | Helicobacter hepaticus | 1,4-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 2 ferricyanide + NADPH | - |
Helicobacter hepaticus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
1.6.5.10 | menadione + NADPH + H+ | two-electron transfer mechanism from NADPH to quinone | Helicobacter hepaticus | ? + NADP+ | - |
? | |
1.6.5.10 | oxidized dichlorophenolindophenol + NADPH + H+ | - |
Helicobacter hepaticus | reduced dicholorophenolindophenol + NADP+ | - |
? | |
1.6.5.10 | ubiquinone-0 + NADPH + H+ | two-electron transfer mechanism from NADPH to quinone | Helicobacter hepaticus | ubiquinol-0 + NADP+ | - |
? | |
1.6.5.10 | ubiquinone-1 + NADPH + H+ | two-electron transfer mechanism from NADPH to quinone | Helicobacter hepaticus | ubiquinol-1 + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.5.2 | ? | x * 21-22000, SDS-PAGE | Helicobacter hepaticus |
1.6.5.10 | ? | x * 21000-22000, SDS-PAGE | Helicobacter hepaticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.5.2 | MdaB | - |
Helicobacter hepaticus |
1.6.5.2 | NADPH quinone reductase | - |
Helicobacter hepaticus |
1.6.5.10 | MdaB | - |
Helicobacter hepaticus |
1.6.5.10 | NADPH quinone reductase | - |
Helicobacter hepaticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.6.5.10 | 22 | - |
assay at room temperature | Helicobacter hepaticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.5.10 | 7.5 | - |
assay at | Helicobacter hepaticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.5.2 | NADH | - |
Helicobacter hepaticus | |
1.6.5.2 | NADPH | - |
Helicobacter hepaticus | |
1.6.5.10 | flavin | flavoprotein, adsorption spectrum with a major peak at 456 nm | Helicobacter hepaticus | |
1.6.5.10 | NADPH | NADH could also be used as an electron donor to MdaB but with much lower efficiency, about 3% of those with NADPH | Helicobacter hepaticus |