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Literature summary extracted from

  • Spreer, A.; Ruechel, R.; Reichard, U.
    Characterization of an extracellular subtilisin protease of Rhizopus microsporus and evidence for its expression during invasive rhinoorbital mycosis (2006), Med. Mycol., 44, 723-731.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.62 medicine expression during non-fatal human rhinoorbital mucormycosis Rhizopus microsporus var. rhizopodiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 alpha2-Macroglobulin inhibits alkaline proteolytic activity of purified Arp by 80% Rhizopus microsporus var. rhizopodiformis
3.4.21.62 additional information alkaline proteolytic activity of purified Arp not inhibited by 3,4-dichloroisocumarin, tosyl lysine chloromethyl ketone, tosyl phenylalanine chloromethyl ketone, leupeptin, chymostatin, elastatinal, iodoacetamide, E-64, EDTA, 1,10-phenanthroline, aprotinin, antithrombin III, alpha1-antitrypsin and soybean trypsin inhibitor Rhizopus microsporus var. rhizopodiformis
3.4.21.62 pepstatin almost completely inhibits acid proteolytic activity, does not inhibit alkaline proteolytic activity of purified Arp Rhizopus microsporus var. rhizopodiformis
3.4.21.62 phenylmethylsulfonyl fluoride inhibits alkaline proteolytic activity of purified Arp by 100%, does not inhibit acid proteolytic activity Rhizopus microsporus var. rhizopodiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.62 33000
-
 x * 33000, SDS-PAGE Rhizopus microsporus var. rhizopodiformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Rhizopus microsporus var. rhizopodiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 to homogeneity by ion exchange chromatography and gel filtration. Alkaline proteolytic activity enriched 1480fold with a yield of 27% Rhizopus microsporus var. rhizopodiformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.62 0.47
-
 culture supernatant Rhizopus microsporus var. rhizopodiformis
3.4.21.62 696
-
 enzyme purified 1480fold Rhizopus microsporus var. rhizopodiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 azocasein + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
3.4.21.62 Bovine serum albumin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
3.4.21.62 casein + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
3.4.21.62 Elastin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
3.4.21.62 Hemoglobin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.62 ? x * 33000, SDS-PAGE Rhizopus microsporus var. rhizopodiformis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 Arp
-
 Rhizopus microsporus var. rhizopodiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.62 55
-
-
 Rhizopus microsporus var. rhizopodiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.62 55 85
-
 Rhizopus microsporus var. rhizopodiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.62 10.5
-
-
 Rhizopus microsporus var. rhizopodiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.62 5.5 12
-
 Rhizopus microsporus var. rhizopodiformis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.62 Rhizopus microsporus var. rhizopodiformis determined in granulated dextran gels
-
 8.8