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Literature summary extracted from

  • Zhang, Y.; Maley, F.; Maley, G.F.; Duncan, G.; Dunigan, D.D.; Van Etten, J.L.
    Chloroviruses encode a bifunctional dCMP-dCTP deaminase that produces two key intermediates in dTTP formation (2007), J. Virol., 81, 7662-7671.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 dCTP activates dCMP deamination about 7fold at 0.005-0.1 mM Paramecium bursaria Chlorella virus 1
3.5.4.13 dCTP activates dCMP deamination about 7fold at 0.005-0.1 mM Paramecium bursaria Chlorella virus 1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.12 ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis Paramecium bursaria Chlorella virus 1
3.5.4.13 ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis Paramecium bursaria Chlorella virus 1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP inhibits dCMP deamination, feedback inhibition Paramecium bursaria Chlorella virus 1
3.5.4.12 H4dUMP inhibits both dCTP and dCMP deaminase activities Paramecium bursaria Chlorella virus 1
3.5.4.12 PDRP inhibits both dCTP and dCMP deaminase activities Paramecium bursaria Chlorella virus 1
3.5.4.13 dTTP inhibits dCMP deamination, feedback inhibition Paramecium bursaria Chlorella virus 1
3.5.4.13 H4dUMP inhibits both dCTP and dCMP deaminase activities Paramecium bursaria Chlorella virus 1
3.5.4.13 PDRP inhibits both dCTP and dCMP deaminase activities Paramecium bursaria Chlorella virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.12 0.64
-
 dCTP pH 9.5, 42°C Paramecium bursaria Chlorella virus 1
3.5.4.12 0.76
-
 dCMP pH 9.5, 42°C, in presence of 0.1 mM dCTP Paramecium bursaria Chlorella virus 1
3.5.4.13 0.64
-
 dCTP pH 9.5, 42°C Paramecium bursaria Chlorella virus 1
3.5.4.13 0.76
-
 dCMP pH 9.5, 42°C, in presence of 0.1 mM dCTP Paramecium bursaria Chlorella virus 1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Ca2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.12 Mg2+ the enzyme depends on divalent cations, Mg2+ is preferred, but can partially be substituted by Ca2+, Ni2+, or Mn2+ Paramecium bursaria Chlorella virus 1
3.5.4.12 Mn2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.12 Ni2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.12 Zn2+ the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation Paramecium bursaria Chlorella virus 1
3.5.4.13 Ca2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.13 Mg2+ the enzyme depends on divalent cations, Mg2+ is preferred , but can partially be substituted by Ca2+, Ni2+, or Mn2+ Paramecium bursaria Chlorella virus 1
3.5.4.13 Mn2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.13 Ni2+ can partially substitute for Mg2+ Paramecium bursaria Chlorella virus 1
3.5.4.13 Zn2+ the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation Paramecium bursaria Chlorella virus 1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.12 17000
-
 6 * 17000, SDS-PAGE Paramecium bursaria Chlorella virus 1
3.5.4.13 17000
-
 6 * 17000, SDS-PAGE Paramecium bursaria Chlorella virus 1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Paramecium bursaria Chlorella virus 1
-
 dUMP + NH3 dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis ?
3.5.4.12 dCTP + H2O Paramecium bursaria Chlorella virus 1
-
 dUTP + NH3
-
 ?
3.5.4.13 dCMP + H2O Paramecium bursaria Chlorella virus 1
-
 dUMP + NH3 dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis ?
3.5.4.13 dCTP + H2O Paramecium bursaria Chlorella virus 1
-
 dUTP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Paramecium bursaria Chlorella virus 1 O41078 i.e. PBCV-1 , a chlorovirus from host Chlorella sp. strain NC64A
-
3.5.4.13 Paramecium bursaria Chlorella virus 1 O41078 i.e. PBCV-1 , a chlorovirus from host Chlorella sp. strain NC64A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml Paramecium bursaria Chlorella virus 1
3.5.4.13 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml Paramecium bursaria Chlorella virus 1

Storage Stability

EC Number Storage Stability Organism
3.5.4.12 the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol Paramecium bursaria Chlorella virus 1
3.5.4.13 the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol Paramecium bursaria Chlorella virus 1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 dCMP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUMP + NH3 dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis ?
3.5.4.12 dCTP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUTP + NH3
-
 ?
3.5.4.12 additional information the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations Paramecium bursaria Chlorella virus 1 ?
-
 ?
3.5.4.13 dCMP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUMP + NH3
-
 ?
3.5.4.13 dCMP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUMP + NH3 dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis ?
3.5.4.13 dCTP + H2O
-
 Paramecium bursaria Chlorella virus 1 dUTP + NH3
-
 ?
3.5.4.13 additional information the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations Paramecium bursaria Chlorella virus 1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 * 17000, SDS-PAGE Paramecium bursaria Chlorella virus 1
3.5.4.13 hexamer 6 * 17000, SDS-PAGE Paramecium bursaria Chlorella virus 1

Synonyms

EC Number Synonyms Comment Organism
3.5.4.12 dCMP-dCTP deaminase
-
 Paramecium bursaria Chlorella virus 1
3.5.4.13 dCMP-dCTP deaminase
-
 Paramecium bursaria Chlorella virus 1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.12 42
-
-
 Paramecium bursaria Chlorella virus 1
3.5.4.13 42
-
-
 Paramecium bursaria Chlorella virus 1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.4.12 25 55
-
 Paramecium bursaria Chlorella virus 1
3.5.4.13 25 55
-
 Paramecium bursaria Chlorella virus 1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.12 5.7
-
 dCTP pH 9.5, 42°C Paramecium bursaria Chlorella virus 1
3.5.4.12 24.9
-
 dCMP pH 9.5, 42°C, in presence of 0.1 mM dCTP Paramecium bursaria Chlorella virus 1
3.5.4.13 5.7
-
 dCTP pH 9.5, 42°C Paramecium bursaria Chlorella virus 1
3.5.4.13 24.9
-
 dCMP pH 9.5, 42°C, in presence of 0.1 mM dCTP Paramecium bursaria Chlorella virus 1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.12 7
-
 dCTP deaminase activity Paramecium bursaria Chlorella virus 1
3.5.4.12 9.5
-
 dCMP deaminase activity Paramecium bursaria Chlorella virus 1
3.5.4.13 7
-
 dCTP deaminase activity Paramecium bursaria Chlorella virus 1
3.5.4.13 9.5
-
 dCMP deaminase activity Paramecium bursaria Chlorella virus 1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.12 6 11 activity range Paramecium bursaria Chlorella virus 1
3.5.4.13 6 11 activity range Paramecium bursaria Chlorella virus 1