EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.16.2 | enzyme expression by in vitro transcription-translation using a rapid translation system with enhanced Escherichia coli lysate and radio-labeled L-Met, tetrahydrobioterin increases the enzyme protein yield | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.16.2 | L205P | the mutant is associated with recessively inherited L-DOPA-responsive infantile parkinsonism, the mutation reduces the activity and stability of the protein in cells and in vitro expression systems, being considered a misfolding mutation | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
1.14.16.2 | binding of inhibitor dopamine and of cofactor tetrahydrobioterin stabilizes the enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | i.e. BH4, cofactor, complex regulation by the cofactor including both enzyme inactivation and conformational stabilization, competitive inhibition, synergistically with DTT | Homo sapiens | |
1.14.16.2 | dopamine | feedback inhibitor and catecholamine product, binding inhibits and stabilizes the enzyme, reversible and competitive inhibition with respect to tetrahydrobiopterin | Homo sapiens | |
1.14.16.2 | DTT | inactivates the enzyme, synergistically with tetrahydrobiopterin | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.2 | additional information | - |
additional information | both the cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin and the feedback inhibitor dopamine increase the kinetic stability of human isozyme 1 in vitro | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 58000 | - |
x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | Homo sapiens | rate-limiting enzyme in the synthesis of catecholamine neurotransmitters, complex regulation by the cofactor including both enzyme inactivation and conformational stabilization, enzyme defects are associated with L-DOPA responsive and non-responsive dystonia and infantile parkinsonism, overview | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.2 | Homo sapiens | - |
isozyme TH 1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.16.2 | brain | - |
Homo sapiens | - |
1.14.16.2 | neuron | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | rate-limiting enzyme in the synthesis of catecholamine neurotransmitters, complex regulation by the cofactor including both enzyme inactivation and conformational stabilization, enzyme defects are associated with L-DOPA responsive and non-responsive dystonia and infantile parkinsonism, overview | Homo sapiens | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.16.2 | ? | x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.16.2 | tyrosine hydroxylase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 25 | - |
assay at, recombinant enzyme | Homo sapiens |
1.14.16.2 | 30 | - |
assay at, crude brain enzyme extract | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 7 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | i.e. BH4, complex regulation by the cofactor including both enzyme inactivation and conformational stabilization, competitive inhibition | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.2 | 0.07 | - |
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | pH 7.0, 25°C, versus (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | Homo sapiens |