EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | Ag+ | 1.5% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | Al3+ | 0.3% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | Co2+ | 46.3% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | Cu2+ | 13.4% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | dithiothreitol | 55% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | EDTA | 87% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | Fe3+ | 5% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | Hg2+ | complete loss of activity at 1 mM | Fusarium solani | |
3.5.5.1 | iodoacetamide | 5% residual activity at 4.6 mM | Fusarium solani | |
3.5.5.1 | Mg2+ | 46% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | NaN3 | 38% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | Ni2+ | 7% residual activity at 5 mM | Fusarium solani | |
3.5.5.1 | p-hydroxymercuribenzoate | 12% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | Pb2+ | 6% residual activity at 1 mM | Fusarium solani | |
3.5.5.1 | Zn2+ | 6% residual activity at 5 mM | Fusarium solani |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.1 | 1.4 | - |
4-cyanopyridine | - |
Fusarium solani | |
3.5.5.1 | 1.5 | - |
Benzonitrile | - |
Fusarium solani | |
3.5.5.1 | 3.2 | - |
3-Cyanopyridine | - |
Fusarium solani |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | Cr3+ | 4% increased activity at 1 mM | Fusarium solani |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 40000 | - |
x * 40000, gel filtration | Fusarium solani |
3.5.5.1 | 580000 | - |
gel filtration | Fusarium solani |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.5.5.1 | 2-propanol | the enzyme shows more than 30% of the control activity with 5% 2-propanol | Fusarium solani |
3.5.5.1 | Acetone | the enzyme shows more than 30% of the control activity with 5% acetone | Fusarium solani |
3.5.5.1 | acetonitrile | the enzyme shows more than 30% of the control activity with 5% acetonitrile | Fusarium solani |
3.5.5.1 | dichloromethane | the enzyme shows more than 30% of the control activity with 5% dichloromethane | Fusarium solani |
3.5.5.1 | dimethylsulfoxide | the enzyme shows more than 30% of the control activity with 5-10% of dimethylsulfoxide | Fusarium solani |
3.5.5.1 | Ethanol | more than half of activity is preserved at up to 15% of ethanol | Fusarium solani |
3.5.5.1 | Ethyl acetate | the enzyme shows more than 30% of the control activity with 5% ethyl acetate | Fusarium solani |
3.5.5.1 | Methanol | the enzyme shows more than 30% of the control activity with 5-10% of methanol | Fusarium solani |
3.5.5.1 | n-heptane | more than half of activity is preserved at up to 50% of n-heptane | Fusarium solani |
3.5.5.1 | n-hexane | more than half of activity is preserved at up to 50% of n-hexane | Fusarium solani |
3.5.5.1 | toluene | the enzyme shows more than 30% of the control activity with 5-10% of toluene | Fusarium solani |
3.5.5.1 | xylene | more than half of activity is preserved at up to 15% of xylene, the enzyme shows more than 30% of the control activity with 5-10% of xylene | Fusarium solani |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Fusarium solani | - |
- |
- |
3.5.5.1 | Fusarium solani O1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.1 | Hi-Prep 16/10 Phenyl FF column chromatography, Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration | Fusarium solani |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.5.1 | mycelium | - |
Fusarium solani | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.5.1 | -18°C, in presence of 2% (w/v) sucrose as a stabilizer, 2 months, 8% loss of activity | Fusarium solani |
3.5.5.1 | -80°C, in presence of 2% (w/v) sucrose as a stabilizer, 2 months, no significant loss of activity | Fusarium solani |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | 3-chlorobenzonitrile + H2O | 87% activity compared to benzonitrile | Fusarium solani | 3-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 3-chlorobenzonitrile + H2O | 87% activity compared to benzonitrile | Fusarium solani O1 | 3-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 3-cyanopyridine + 2 H2O | 28% activity compared to benzonitrile | Fusarium solani | 3-pyridinecarboxylic acid + NH3 | - |
? | |
3.5.5.1 | 3-hydroxybenzonitrile + H2O | 80% activity compared to benzonitrile | Fusarium solani | 3-hydroxybenzoic acid + NH3 | - |
? | |
3.5.5.1 | 3-tolunitrile + H2O | 33% activity compared to benzonitrile | Fusarium solani | 3-methylbenzoate + NH3 | - |
? | |
3.5.5.1 | 4-aminobenzonitrile + H2O | 7% activity compared to benzonitrile | Fusarium solani | 4-aminobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 4-chlorobenzonitrile + H2O | 40% activity compared to benzonitrile | Fusarium solani | 4-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 4-chlorobenzonitrile + H2O | 40% activity compared to benzonitrile | Fusarium solani O1 | 4-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 4-cyanopyridine + 2 H2O | 130% activity compared to benzonitrile | Fusarium solani | 4-pyridinecarboxylic acid + NH3 | - |
? | |
3.5.5.1 | 4-cyanopyridine + 2 H2O | 130% activity compared to benzonitrile | Fusarium solani O1 | 4-pyridinecarboxylic acid + NH3 | - |
? | |
3.5.5.1 | 4-hydroxybenzonitrile + H2O | 3% activity compared to benzonitrile | Fusarium solani | 4-hydroxybenzoic acid + NH3 | - |
? | |
3.5.5.1 | 4-tolunitrile + H2O | 16% activity compared to benzonitrile | Fusarium solani | 4-methylbenzoate + NH3 | - |
? | |
3.5.5.1 | benzonitrile + 2 H2O | 100% activity | Fusarium solani | benzoic acid + NH3 | - |
? | |
3.5.5.1 | benzonitrile + 2 H2O | 100% activity | Fusarium solani O1 | benzoic acid + NH3 | - |
? | |
3.5.5.1 | butyronitrile + H2O | 20% activity compared to benzonitrile | Fusarium solani | butyric acid + NH3 | - |
? | |
3.5.5.1 | isobutyronitrile + H2O | 4% activity compared to benzonitrile | Fusarium solani | isobutyric acid + NH3 | - |
? | |
3.5.5.1 | lactonitrile + H2O | 4% activity compared to benzonitrile | Fusarium solani | lactic acid + NH3 | - |
? | |
3.5.5.1 | methacrylonitrile + H2O | 14% activity compared to benzonitrile | Fusarium solani | methylacrylic acid + NH3 | - |
? | |
3.5.5.1 | methacrylonitrile + H2O | 14% activity compared to benzonitrile | Fusarium solani O1 | methylacrylic acid + NH3 | - |
? | |
3.5.5.1 | propionitrile + H2O | 18% activity compared to benzonitrile | Fusarium solani | propionic acid + NH3 | - |
? | |
3.5.5.1 | valeronitrile + H2O | 26% activity compared to benzonitrile | Fusarium solani | valeric acid + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.1 | multimer | x * 40000, gel filtration | Fusarium solani |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 40 | 45 | - |
Fusarium solani |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 35 | 55 | the nitrilase half-life is 277 min and 10.5 h at 35°C and 45°C, respectively, the enzyme is active at up to 50°C, the enzyme shows only traces of activity at 55°C | Fusarium solani |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 8 | - |
- |
Fusarium solani |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 5 | 10 | - |
Fusarium solani |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 5 | 11 | the enzyme shows 5%, 39% and 29% of the maximum activity at pH 5, 6 and 10, respectively, and it is nearly inactive at pH 11 | Fusarium solani |