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Literature summary extracted from

  • Xie, Z.; Feng, J.; Garcia, E.; Bernett, M.; Yazbeck, D.; Tao, J.
    Cloning and optimization of a nitrilase for the synthesis of (3S)-3-cyano-5-methyl hexanoic acid (2006), J. Mol. Catal. B, 41, 75-80.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli strains BL21 (DE3), BRLplys (DE3), Rosetta (DE3), and Tuner (DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 A200G 37% increased activity compared to the wild type enzyme Arabidopsis thaliana
3.5.5.1 C236S mutant has a nearly 3fold increase in reactivity Arabidopsis thaliana
3.5.5.1 H101Q/C239S/D246E 47% increased activity compared to the wild type enzyme Arabidopsis thaliana
3.5.5.1 I29L 113% increased activity compared to the wild type enzyme Arabidopsis thaliana
3.5.5.1 P172S/C236S/V291I 84% increased activity compared to the wild type enzyme Arabidopsis thaliana
3.5.5.1 R89S/G179G 89% increased activity compared to the wild type enzyme Arabidopsis thaliana
3.5.5.1 T28S 30% increased activity compared to the wild type enzyme Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 440000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 Ni-NTA resin chromatography and Superdex 75 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 (3S)-isobutylsuccinonitrile + H2O specific for the (3S)-isomer Arabidopsis thaliana (3S)-3-cyano-5-methyl hexanoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 dodecamer gel filtration Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NIT1
-
 Arabidopsis thaliana