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Literature summary extracted from

  • Martins, M.; Pluvinage, B.; de la Sierra-Gallay, I.L.; Barbault, F.; Dairou, J.; Dupret, J.M.; Rodrigues-Lima, F.
    Functional and structural characterization of the arylamine N-acetyltransferase from the opportunistic pathogen Nocardia farcinica (2008), J. Mol. Biol., 383, 549-560.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.5 medicine there is increasing evidence that arylamine N-acetyltransferases could contribute to antibiotic resistance in pathogenic bacteria Nocardia farcinica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 expressed in Escherichia coli as a His-tagged fusion protein Nocardia farcinica
2.3.1.5 into the pET28a vector for expression in Escherichia coli BL21DE3 pLysS cells Nocardia farcinica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.5 crystal structure of NfNAT is solved at a resolution of 2.7 A. Despite low sequence identity, enzyme shares an almost identical fold with that of other prokaryotic NATs, e.g. Mycobacterium smegmatis NAT and Mycobacterium marinum NAT. The overall NfNAT structure consists of three domains of equivalent size. The first two domains, a helical bundle (amino acids 1-98) and a beta-barrel (amino acids 99-199), are disposed in such a way that three residues (Cys82, His119, and Asp136) form a catalytic triad. The third domain is linked to the second through an interdomain helix (alpha6: amino acids 200-210). As shown for other prokaryotic NAT structures, the interface formed between domains 2 and 3 forms a substantial active-site cleft Nocardia farcinica
2.3.1.5 the structure of NfNAT is solved at a resolution of 2.7 A Nocardia farcinica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 1.414
-
 isoniazid
-
 Nocardia farcinica
2.3.1.5 1.414
-
 isoniazid apparent KM Nocardia farcinica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 36000
-
 SDS-PAGE Nocardia farcinica
2.3.1.5 36000
-
 determined by SDS-PAGE and Western Blot analysis Nocardia farcinica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Nocardia farcinica
-
-
-
2.3.1.5 Nocardia farcinica Q5YYQ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 using HIS-Select resin Nocardia farcinica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 0.013
-
 substrate: 2-aminofluorene Nocardia farcinica
2.3.1.5 0.015
-
 sulfamethazine Nocardia farcinica
2.3.1.5 0.015
-
 substrate: sulfamethazine Nocardia farcinica
2.3.1.5 0.023
-
 substrate: beta-naphthylamine Nocardia farcinica
2.3.1.5 0.024
-
 substrate: 4-aminobiphenyl Nocardia farcinica
2.3.1.5 0.02463
-
 substrate: 5-aminosalicylate Nocardia farcinica
2.3.1.5 0.029
-
 substrate: sulfamethoxazole Nocardia farcinica
2.3.1.5 0.029
-
 sulfamethoxazole Nocardia farcinica
2.3.1.5 0.052
-
 substrate: benzidine Nocardia farcinica
2.3.1.5 0.079
-
 substrate: 4-aminoveratrole Nocardia farcinica
2.3.1.5 0.087
-
 substrate: hydralazine Nocardia farcinica
2.3.1.5 0.111
-
 4-aminobenzoic acid Nocardia farcinica
2.3.1.5 0.111
-
 substrate: 4-aminobenzoic acid Nocardia farcinica
2.3.1.5 0.372
-
 4-aminosalicylate Nocardia farcinica
2.3.1.5 0.372
-
 substrate: 4-aminosalicylate Nocardia farcinica
2.3.1.5 2.878
-
 isoniazid Nocardia farcinica
2.3.1.5 2.878
-
 substrate: isoniazid Nocardia farcinica
2.3.1.5 13.03
-
 4-aminofluorene Nocardia farcinica
2.3.1.5 23.73
-
 beta-naphthylamine Nocardia farcinica
2.3.1.5 24.63
-
 5-aminosalicylate Nocardia farcinica
2.3.1.5 24.91
-
 2-aminobiphenyl Nocardia farcinica
2.3.1.5 52.33
-
 benzidine Nocardia farcinica
2.3.1.5 79.39
-
 4-aminoveratrole Nocardia farcinica
2.3.1.5 87.76
-
 hydralazine Nocardia farcinica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-aminobiphenyl
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + 2-aminofluorene
-
 Nocardia farcinica CoA + N-acetyl-2-aminofluorene
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid
-
 Nocardia farcinica CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobiphenyl
-
 Nocardia farcinica CoA + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 acetyl-CoA + 4-aminofluorene
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + 4-aminosalicylate
-
 Nocardia farcinica CoA + N-acetyl-4-aminosalicylate
-
 ?
2.3.1.5 acetyl-CoA + 4-aminosalicylate
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + 4-aminoveratrole
-
 Nocardia farcinica CoA + N-acetyl-4-aminoveratrole
-
 ?
2.3.1.5 acetyl-CoA + 4-aminoveratrole
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylate
-
 Nocardia farcinica CoA + N-acetyl-5-aminosalicylate
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylate
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + benzidine
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + benzidine
-
 Nocardia farcinica CoA + N-acetyl-benzidine
-
 ?
2.3.1.5 acetyl-CoA + beta-naphthylamine
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + beta-naphthylamine
-
 Nocardia farcinica CoA + N-acetyl-beta-naphthylamine
-
 ?
2.3.1.5 acetyl-CoA + hydralazine
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + hydralazine
-
 Nocardia farcinica CoA + N-acetyl-hydralazine
-
 ?
2.3.1.5 acetyl-CoA + isoniazid
-
 Nocardia farcinica CoA + N-acetyl-isoniazid
-
 ?
2.3.1.5 acetyl-CoA + isoniazid
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Nocardia farcinica ?
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Nocardia farcinica CoA + N-acetyl-sulfamethazine
-
 ?
2.3.1.5 acetyl-CoA + sulfamethoxazole
-
 Nocardia farcinica CoA + N-acetyl-sulfamethoxazole
-
 ?
2.3.1.5 acetyl-CoA + sulfamethoxazole
-
 Nocardia farcinica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Nocardia farcinica
2.3.1.5 NAT
-
 Nocardia farcinica
2.3.1.5 NfNAT
-
 Nocardia farcinica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 37
-
 assay at Nocardia farcinica
2.3.1.5 37
-
 activity assay Nocardia farcinica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7.5
-
 assay at Nocardia farcinica
2.3.1.5 7.5
-
 activity assay Nocardia farcinica

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Nocardia farcinica