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Literature summary extracted from

  • Chintalapati, S.; Al Kurdi, R.; van Scheltinga, A.C.; Kuehlbrandt, W.
    Membrane structure of CtrA3, a copper-transporting P-type-ATPase from Aquifex aeolicus (2008), J. Mol. Biol., 378, 581-595.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.8 L-cysteine isoform CtrA2 shows highest activity at 20 mM L-cysteine, isoform CtrA3 shows highest activity at 15 mM L-cysteine Aquifex aeolicus
7.2.2.8 additional information isoform CtrA2 is activated without added lipid, whereas the activity of isoform CtrA3 depended on the addition of extraneous lipid. Escherichia coli polar lipids, soybean phosphatidyl choline, synthetic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and a total extract of Aquifex aeolicus lipids all have roughly the same effect Aquifex aeolicus
7.2.2.8 NaCl highest activity at about 400 mM NaCl Aquifex aeolicus
7.2.2.9 cysteine stimulated by, optimum concentration of 15 mM for CtrA3 Aquifex aeolicus
7.2.2.9 cysteine stimulated by, optimum concentration of 20 mM Aquifex aeolicus
7.2.2.9 additional information activity depends on addition of extraneous lipid, similar effects for polar lipids of Escherichia coli, phosphatidylcholine of Glycine max, total extract of Aquifex aeolicus lipids and for the synthetic lipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine Aquifex aeolicus
7.2.2.9 NaCl activity stimulated by, plateau at 400 mM Aquifex aeolicus
7.2.2.9 NaCl CtrA2 and CtrA3, stimulated by, plateau at 400 mM Aquifex aeolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.8 expressed in Escherichia coli BL21(DE3) cells Aquifex aeolicus
7.2.2.9 expression in Escherichia coli Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.2.2.8
-
 Aquifex aeolicus
7.2.2.9 electron cryomicroscopy of two-dimensional crystals, projection map at 7 A resolution with density peaks Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.9 thiol activity of CtrA3 decreased at concentrations of 20 mM and above Aquifex aeolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.9 membrane
-
 Aquifex aeolicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.8 Ag+ isoform CtrA2 and CtrA3 are activated by Ag+ (with Ag+ being twice as effective as Cu+ in case of isoform CtrA2) Aquifex aeolicus
7.2.2.8 Cu+ isoform CtrA2 is activated by Cu+ Aquifex aeolicus
7.2.2.8 Cu2+ isoform CtrA3 is activated by Cu2+ Aquifex aeolicus
7.2.2.8 additional information not activated by Zn2+, Ni2+, Mg2+ or Co2+ Aquifex aeolicus
7.2.2.9 Ag+ purified CtrA2 protein, stimulation twice as efficient as by Cu2+ Aquifex aeolicus
7.2.2.9 Ag+ purified CtrA3 protein, stimulation twice as efficient as by Cu2+ Aquifex aeolicus
7.2.2.9 Co2+ activity slightly above background as measured in the absence of any such ion Aquifex aeolicus
7.2.2.9 Cu2+ purified CtrA2 protein, stimulated by Cu2+ in the presence of DTT, not stimulated by Cu2+ Aquifex aeolicus
7.2.2.9 Cu2+ purified CtrA3 protein, stimulated by Cu2+, no stimulation by Cu2+ in the presence of DTT Aquifex aeolicus
7.2.2.9 Mg2+ activity slightly above background as measured in the absence of any such ion Aquifex aeolicus
7.2.2.9 Ni2+ activity slightly above background as measured in the absence of any such ion Aquifex aeolicus
7.2.2.9 Zn2+ activity slightly above background as measured in the absence of any such ion Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.8 78000
-
 x * 78000, isoform CtrA3, SDS-PAGE Aquifex aeolicus
7.2.2.8 80000
-
 x * 80000, isoform CtrA2, SDS-PAGE Aquifex aeolicus
7.2.2.9 75000
-
 CtrA3 protein, SDS-PAGE Aquifex aeolicus
7.2.2.9 80000
-
 SDS-PAGE Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1] Aquifex aeolicus
-
 ADP + phosphate + Cu+[side 2]
-
 ?
7.2.2.9 ATP + H2O + Cu2+/in Aquifex aeolicus
-
 ADP + phosphate + Cu2+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.8 Aquifex aeolicus
-
-
-
7.2.2.9 Aquifex aeolicus O67203
-
-
7.2.2.9 Aquifex aeolicus O67432
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.8 Ni2+-NTA column chromatography Aquifex aeolicus
7.2.2.9 gel filtration Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.9 additional information
-
 CtrA2 protein shown to have a CPC metal-binding sequence in TM6 and a CxxC metal-binding N-terminal domain, substrate ion-induced activity on lipids, pH, temperature, ionic strength and thiols tested, CtrA2 protein activated by Ag+ and Cu2+, transport of reduced copper ion Aquifex aeolicus
7.2.2.9 additional information
-
 CtrA3 protein has a CPH metal-binding motif in TM6 and a histidine-rich N-terminal metal-binding domain substrate ion-induced activity on lipids, pH, temperature, ionic strength and thiols, eight membrane-spanning alpha-helices per monomer determined by structural analysis, arrangement of the six central helices surrounding the ion-binding site in the membrane conserved, sequence alignment and fitting of domains to the projection map Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1]
-
 Aquifex aeolicus ADP + phosphate + Cu+[side 2]
-
 ?
7.2.2.9 ATP + H2O + Cu2+/in
-
 Aquifex aeolicus ADP + phosphate + Cu2+/out
-
 ?
7.2.2.9 ATP + H2O + Cu2+/in cloning, expression and structural characterization of copper-transporting ATPase CtrA2 Aquifex aeolicus ADP + phosphate + Cu2+/out
-
 ?
7.2.2.9 ATP + H2O + Cu2+/in cloning, expression and structural characterization of copper-transporting ATPase CtrA3 Aquifex aeolicus ADP + phosphate + Cu2+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.8 ? x * 78000, isoform CtrA3, SDS-PAGE Aquifex aeolicus
7.2.2.8 ? x * 80000, isoform CtrA2, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 copper-transporting P-type-ATPase
-
 Aquifex aeolicus
7.2.2.8 CtrA2 isoform Aquifex aeolicus
7.2.2.8 CtrA3 isoform Aquifex aeolicus
7.2.2.9 CtrA2 protein
-
 Aquifex aeolicus
7.2.2.9 CtrA3 protein
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.8 75
-
-
 Aquifex aeolicus
7.2.2.9 75
-
 thermophilic protein, activity maximum at Aquifex aeolicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.2.2.9 20 90 maximum at 75°C, decreased to between 50% and 70% of this value at 90°C, significantly active down to 50°C Aquifex aeolicus
7.2.2.9 20 90 maximum at 75°C, shown to be decreased to between 50% and 70% of this value at 90°C, shown to be completely inactive at 50°C or below Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.8 7
-
-
 Aquifex aeolicus
7.2.2.9 7
-
 activity of CtrA3 stimulated by Cu2+ Aquifex aeolicus
7.2.2.9 7
-
 CtrA2 protein stimulated by Ag+ Aquifex aeolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.2.2.9 5 9 ATPase activities stimulated by Ag+ Aquifex aeolicus
7.2.2.9 5 9 ATPase activities stimulated by Cu2+ measured at different pH values Aquifex aeolicus

General Information

EC Number General Information Comment Organism
7.2.2.8 physiological function isoform CtrA2 presumably transports reduced Cu+, while isoform CtrA3 presumably transports the oxidized Cu+ Aquifex aeolicus