BRENDA - Enzyme Database

Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability

Fedoy, A.E.; Yang, N.; Martinez, A.; Leiros, H.K.; Steen, I.H.; J. Mol. Biol. 372, 130-149 (2007)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.42
crystals of native DpIDH are obtained by the sitting-drop, vapour-diffusion method. The crystals are grown at 8C. Crystals of DpIDH in complex with isocitrate (DpIDH-iso) are obtained by the hanging drop method at 8 C
Desulfotalea psychrophila
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Desulfotalea psychrophila
Q6AQ66
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
688373
Desulfotalea psychrophila
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
70
-
melting temperature is 66.9C
Desulfotalea psychrophila
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
4.5
-
isocitrate
5C
Desulfotalea psychrophila
1.1.1.42
4.5
-
NADP+
5C
Desulfotalea psychrophila
1.1.1.42
31.4
-
isocitrate
25C
Desulfotalea psychrophila
1.1.1.42
31.4
-
NADP+
25C
Desulfotalea psychrophila
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.42
crystals of native DpIDH are obtained by the sitting-drop, vapour-diffusion method. The crystals are grown at 8C. Crystals of DpIDH in complex with isocitrate (DpIDH-iso) are obtained by the hanging drop method at 8 C
Desulfotalea psychrophila
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
688373
Desulfotalea psychrophila
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
70
-
melting temperature is 66.9C
Desulfotalea psychrophila
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
4.5
-
isocitrate
5C
Desulfotalea psychrophila
1.1.1.42
4.5
-
NADP+
5C
Desulfotalea psychrophila
1.1.1.42
31.4
-
isocitrate
25C
Desulfotalea psychrophila
1.1.1.42
31.4
-
NADP+
25C
Desulfotalea psychrophila