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Literature summary extracted from

  • Fujiwara, K.; Hosaka, H.; Matsuda, M.; Okamura-Ikeda, K.; Motokawa, Y.; Suzuki, M.; Nakagawa, A.; Taniguchi, H.
    Crystal structure of bovine lipoyltransferase in complex with lipoyl-AMP (2007), J. Mol. Biol., 371, 222-234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.181 for expression in Escherichia coli BL21DE3 pLysS cells Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.181 the crystal structure of lipoyltransferase in complex with lipoyl-AMP is determined at a resolution of 2.1 A Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.181 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + protein Bos taurus
-
 protein N6-(octanoyl)lysine + [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.181 Bos taurus O46419
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.181 hydroxylapatite and DEAE-Sepharose columns are used for the purification of the recombinant protein and for the purification of lipoyltransferase from bovine liver mitochondria Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.181 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + protein
-
 Bos taurus protein N6-(octanoyl)lysine + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.181 bLT
-
 Bos taurus
2.3.1.181 lipoyl(octanoyl) transferase
-
 Bos taurus
2.3.1.181 lipoyltransferase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.181 37
-
 assay of lipoylation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.181 7.8
-
 assay of lipoylation Bos taurus